| Cat.No.: | EL-0403 |
| Product Name: | F9 nuclear extract (Retinoic acid treated) |
| Description: | F9 nuclear extract (Retinoic acid treated) was prepared from cell cultures of the mouse teratocarcinoma F9 stem cell line. This cell line expresses characteristics of early mouse embryonal cells and is considered to be nullipotent because of its inability to spontaneously differentiate. However, introduction of chemical agents, such as retinoic acid (RA), or transfection with oncogenic sequences, such as SV40 large T antigen, can induce differentiation of these cells. F9 cells readily differentiate into an endodermal-like derivative phenotype, but possess the ability to differentiate into derivatives of all three germ layers under the appropriate culture conditions, suggesting that these cells may be pluripotent. The F9 stem cell line is widely used in research to study the biomolecular mechanisms underlying differentiation. |
| Species: | Murine |
| Source: | Murine testes (Embryonal Carcinoma) |
| Components: | 2 x 100 µg of F9 nuclear extract (Retinoic acid treated) at 2.5 µg/µl. |
| Extract Composition: | F9 nuclear extract (Retinoic acid treated) is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). Cells were treated with 1 µM Retinoic acid for 48 hours. Retinoic acid is refreshed after 24 hours. Cells must be grown on plates coated with 0.1% gelatin. |
| Application Notes: | F9 nuclear extract (Retinoic acid treated) is specifically recommended for studies related to differentiation and in stem cell research. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
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| EL-0005 | Recombinant Human MBD5 Cell Lysate | Inquiry |
USA
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