Caco-2 nuclear extract


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Cat.No.:  EL-0409
Product Name:  Caco-2 nuclear extract
Description:  Caco-2 nuclear extract was prepared from cell cultures of the human colorectal adenocarcinoma Caco-2 cell line. The Caco-2 cell line was originally derived from a colon carcinoma and functionally demonstrates properties resembling enterocytes of the small intestine. When confluent, these cells form a polarized epithelial monolayer with properties similar to those of the mucosal lining of the small intestine. In research, Caco-2 cells are most commonly as in vitro models of the small intestinal mucosa to study the physiological and biochemical properties of intestinal permeability, adsorption, transport and metabolism of ions, small molecules, and drug therapies.
Species:  Human
Source:  Human Colorectal Adenocarcinoma
Components:  200 µg of Caco-2 nuclear extract at 7.11 µg/µl.
Extract Composition:  Caco-2 cells are grown to 90% confluence before harvesting' the nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).
Application Notes:  Caco-2 nuclear extract is specifically recommended for analysis of the biophysical and biochemical properties associated with small intestinal mucosa.
Storage:  Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Warning:  For research use only, not for diagnostic or therapeutic use.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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