| Cat.No.: | EL-0413 |
| Product Name: | A-431 nuclear extract |
| Description: | A-431 nuclear extract was prepared from cell cultures of the human epidermoid carcinoma A431 cell line. These cells are characterized by abnormally high levels of expression of epidermal growth factor receptor (EGFR), approximately 3 x 106/cell, on the cell surface and exhibits a biphasic response to varying concentrations of epidermal growth factor (EGF). Specifically, lower (pmol) concentrations of EGF stimulates cell growth while higher (nmol) concentrations inhibits cell growth and induces terminal differentiation. Therefore, these cells are commonly used as a positive control for EGFR expression and in studies of EGFR downstream signaling cascades. These cells are also highly responsive to mitogenic stimuli and are naturally devoid of p53, so are frequently used in cancer and cell cycle-associated studies. |
| Species: | Human |
| Source: | Human Epidermoid Carcinoma |
| Components: | 200 µg of A-431 nuclear extract at 2.5 µg/µl. |
| Extract Composition: | A-431 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). |
| Application Notes: | A-431 nuclear extract is specifically recommended for analysis of 1) EGFR expression and downstream signaling cascades, 2) cell cycle, and 3) cancer-associated signaling pathways. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0001 | Recombinant Human MBD1 Cell Lysate | Inquiry |
| EL-0002 | Recombinant Human MBD3 Cell Lysate | Inquiry |
| EL-0003 | Recombinant Human MBD3L1 293 Cell Lysate | Inquiry |
| EL-0004 | Recombinant Human MBD4 Lysate | Inquiry |
| EL-0005 | Recombinant Human MBD5 Cell Lysate | Inquiry |
USA
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