| Cat.No.: | EL-0414 |
| Product Name: | 293 nuclear extract |
| Description: | 293 nuclear extract was prepared from cell cultures of the Human Embryonic Kidney (HEK) 293 cell line. This cell line was originally derived from transformation of normal human embryonic tissue cultures with a sheared DNA fragment of the left arm of the adenoviral 5 viral genome. These cells are commonly used in research due to their ease of growth and transfectability. However, since the cellular origin of these hypotriploid cells is poorly understood, they are not recommended for use as an in vitro model for kidney cell function. 293 nuclear extract contains all the basal transcriptional and mRNA splicing components and serves as an ideal cell-free platform for analysis of transcription and pre-mRNA processing mechanisms. |
| Species: | Human |
| Source: | Human Embryonic Kidney |
| Components: | 200 µg of 293 nuclear extract at 4.68 µg/µl. |
| Extract Composition: | 293 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). |
| Application Notes: | 293 nuclear extract is specifically recommended for analysis of 1) in vitro transcription, 2) protein-protein and protein-DNA/RNA interactions, and 3) in vitro splicing. |
| Storage: | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Warning: | For research use only, not for diagnostic or therapeutic use. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0001 | Recombinant Human MBD1 Cell Lysate | Inquiry |
| EL-0002 | Recombinant Human MBD3 Cell Lysate | Inquiry |
| EL-0003 | Recombinant Human MBD3L1 293 Cell Lysate | Inquiry |
| EL-0004 | Recombinant Human MBD4 Lysate | Inquiry |
| EL-0005 | Recombinant Human MBD5 Cell Lysate | Inquiry |
USA
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