ChIP-qPCR combines chromatin immunoprecipitation (ChIP) technology with real-time quantitative PCR to study protein-DNA interactions at known genomic binding sites. ChIP technology utilizes antibody specificity to enrich DNA fragments bound to specific transcription factor/histone modification; qPCR technology uses SYBR fluorescent dye, non-specifically incorporating into DNA double strands, emitting fluorescence, to ensure that the increase of fluorescence signal and increased PCR products are completely synchronized. And the finally quantitative analysis is carried out using Ct value. Because of the lowest cost of performing qPCR, ChIP-qPCR is an attractive strategy, which is advantageous for the research of interrogating specific genes and potential regulatory regions in a wide range under different experimental conditions.

Features of ChIP-qPCR

1. High specificity, specific real-time fluorescent qPCR primers designed for gene promoters of interest
2. An ultra-wide linear range spanning seven orders of magnitude
3. High accuracy, good repeatability, and high sensitivity
4. Cost-effective technique

Advantages of Choosing Creative BioMart

1. Tolerance of multiple sample types: we currently accept a wide range of biological and clinical samples including cells and tissues.
2. One-stop service: customer only need to provide samples, and we will complete all the experiment. We provide data analysis on request. The complete report is provided to you with both raw and analyzed data, graphs, detailed protocols, and a summary of findings.
3. Reliable data quality: our state-of-the-art technology and stringent quality control (QC) ensure that our services and the final presentation of data are reliably performed and presented.
4. Professional data analysis: at Creative BioMart, there is professional ChIP-qPCR data processing flow. a) The total input DNA sample is used as the template, and the specific primers of the gene to be tested are used for qPCR detection to obtain Ct value (input); b) The ChIP DNA sample is detected as a template to obtain Ct value (ChIP); c) The efficiency of co-immunoprecipitation of the detected gene (ChIP / Total input) can be calculated by the following formula: %( ChIP / Total input) = 2^[ Ct (Input) – Ct (ChIP) ] × 100.

Workflow of ChIP-qPCR at Creative BioMart

Figure 1. Workflow of ChIP-qPCR at Creative BioMart

As one of the leading biotechnology company in the world, Creative BioMart has the extensive experience and expertise to provide you high-quality ChIP-qPCR service. Creative BioMart’s goal is to provide you with efficient, reliable and cost-effective solutions to accelerate your epigenetics research. contact us for more information about our ChIP-qPCR service.