By integrating chromatin immunoprecipitation and next-generation sequencing technology (ChIP-Seq), the genome-wide landscape of regulatory elements that control specific genomes can now be thoroughly explored. This led to the discovery of a novel subpopulation of specific enhancers called super-enhancers, which are large enhancer clusters that cover extremely long regulatory DNA regions and are densely occupied by transcription factors, active histone marks, and coactivators. There is growing evidence that super-enhancer plays a key role in cell type-specific development and differentiation as well as in the development of various diseases. Creative BioMart provides our customers with super-enhancer profiling service to help you complete genome-wide super-enhancer screening, which may be beneficial for disease diagnosis and the development of clinical therapeutics designed to target super-enhancers.

Figure 1. Comparison of super-enhancers and typical enhancers (Shin H Y. 2018)

Advantages of Choosing Creative BioMart

1. Skillful technical team & First-class technology platform: Creative BioMart has years of experience in ChIP-Seq, and our state-of-art technologies and strict quality control at every step make sure that our services and final presented data are performed and presented with reliability.
2. One-stop solution: Creative BioMart offers one-stop solutions to all your custom research needs. Customers only need to provide samples, and we will complete full super-enhancer profiling experiment from cell preparation, chromatin isolation and sonication, ChIP with a ChIP-qualified antibody, ChIP-Seq libraries construction, on-machine sequencing to bioinformatics analysis. The complete report is provided to you with both raw and analyzed data, graphs, detailed protocols, and a summary of findings.
3. Reliable data quality & Professional bioinformatics analysis: our data quality guarantee, as measured by the percentage of bases with a sequencing quality score above Q30 (PE 150, ≥80%; SE 50, ≥90%), exceeds Illumina’s official guarantee (PE 150, ≥75%; SE 50, ≥80%). In addition, we have organized a professional bioinformatics analysis technical team. We have comprehensive hardware equipment, data analysis pipelines and analytical tools specifically designed for super-enhancer profiling.

Workflow of Super-enhancer Profiling at Creative BioMart

Figure 2. Workflow of super-enhancer profiling service at Creative BioMart

Creative BioMart is dedicated to ensuring all services on epigenetics are at the highest level of quality. With years of know-how, our experienced scientists will work closely with you to provide help in super-enhancer profiling. Creative BioMart is a reliable partner that can help you with greatest chance to succeed. contact us for more information about our super-enhancer profiling service.

Reference
1. Shin H Y. Targeting super-enhancers for disease treatment and diagnosis. Molecules & Cells. 2018, 41(6): 506-514.