Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-Seq) has been a powerful tool for studying the status of histone modification. Histone ChIP-Seq is sensitive to the histone content of chromatin, especially to the incorporation of specific post-translational histone modifications in chromatin. This technique is widely used in stem cell research and understanding disease progression. It can also be utilized to identify novel biomarkers, because histone modification patterns are capable of predicting gene expression and can therefore be detected prior to changes in gene expression. Creative BioMart offers customers histone ChIP-Seq service, which provides accurate and repeatable identification for histone modifications in the genome with high sensitivity and specificity.

Advantages of Choosing Creative BioMart

1. Skillful technical team & Advanced technology platform: with years of experience in ChIP-Seq services, Creative BioMart is dedicated to making our cutting-edge histone ChIP-Seq assay accessible to a wider life science community. Our state-of-art technologies and strict quality control at every step make sure that our services and final presented data are performed and presented with reliability.
2. Flexible and one-stop service: Creative BioMart offers flexible and one-stop services to all your custom research needs. Customers only need to provide samples, and we will complete full histone ChIP-Seq experiment. After discussion with our customers about the goal, options and implications, customized assay development and specific assay are also supported at a reasonable cost.
3. Professional data analysis: Creative BioMart performs an integrative or individual analysis for chromatin with the ENCODE (the encyclopedia of DNA elements, a public research project) standards, using different data analysis pipelines to study different types of protein-chromatin interactions. The data analysis pipelines of transcription factor ChIP-Seq and histone ChIP-Seq share the same mapping steps but differ in the way the signal and peak are called and in the subsequent statistical treatment of replicates. The histone ChIP-Seq pipeline is suitable for proteins that associate with DNA over longer regions or domains.

Workflow of Histone ChIP-Seq at Creative BioMart

Figure 1. Workflow of histone ChIP-Seq service at Creative BioMart

Creative BioMart provides full support on study design to ensure correct sequencing and bioinformatics strategies are used to meet your project goals. Our expert will consult with you about your specific requirements. Send us your sample and we will send you high-quality data to propel your research forward. Please contact us for more information at any time!