Global DNA 5fC/5caC Quantification by LC-MS/MS


The rapid development of 5-hydroxymethylcytosine (5hmC) assays could guide studies of other nucleotide variants, particularly 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), which have been recently identified in mammalian DNA. In order to better quantify the global levels of 5-methylcytosine (5mC) oxidation derivatives, mass spectrometry (MS)-based methods have been developed. A more accurate method for quantifying low levels of modified nucleotides is liquid chromatography (LC)-mass spectroscopy (MS), which separates and identifies hydrolyzed nucleotides. By combining LC with high-resolution MS and using isotope-labeled internal standard to improve the detection limits and allow for accurate quantification of rare base modifications.

Creative BioMart provides customers with global DNA 5fC/5caC quantification service, which is highly accurate and sensitive, independent of DNA quality and sequence. The measurement results reflect the absolute level of global rare base modifications, but it is not suitable to determine the precise location of these modifications.

Advantages of Choosing Creative BioMart

  1. Tolerance of multiple sample types: we accept a wide range of biological and clinical samples, including but not limited to, genomic DNA isolated from a variety of species, cultured cells, fresh/frozen tissues, formalin-fixed and paraffin-embedded (FFPE) samples.
  2. One-stop service: you only need to provide samples, and Creative BioMart will complete the full LC-MS/MS experiment and data analysis. We will provide a comprehensive and in-depth report, including both raw and analyzed data, graphs, detailed protocols, and a summary of findings.
  3. Advanced technology platform: we have state-of-the-art LC-MS/MS equipment, and our technical team has accumulated years of expertise and experience in complicated MS specifications.
  4. Competitive pricing & Short turnaround time: our service is efficient and reliable with a relatively low cost. Creative BioMart is committed to long-term partnerships, so services provided by us have competitive price and short turnaround time.

Workflow of Our Service at Creative BioMart

Workflow of Global DNA 5fC/5caC Quantification by LC-MS/MS at Creative BioMart

Figure 1. Workflow of Global DNA 5fC/5caC Quantification by LC-MS/MS at Creative BioMart

Creative BioMart has worked in this field for years and has extensive experience in epigenetics research. We are dedicated to providing data of the highest quality for our customers and helping accelerate the epigenetic research. Do not hesitate to contact us for more information.

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