Chromatin interaction analysis by paired-end tag (ChIA-PET) sequencing is one of the chromosome conformation capture (3C) technique-derived approaches, and ChIA-PET is unique compared to the high-through chromosome conformation capture (Hi-C) technique, which allows specific protein factors and their associated chromatin interactions to be interrogated at high resolution within the genome. At Creative BioMart, we provide our customers with high-quality ChIA-PET service to accelerate your research.

What Is CHIA-PET Sequencing?

ChIA-PET sequencing perfectly combines chromatin immunoprecipitation (ChIP) with 3C technology, allowing identification of all of the contacts from all of the sites bound by a protein of interest. In this technique, the sample is first cross-linked and then sonicated, followed by ChIP to enrich the DNA-protein complexes. DNA linkers are then ligated to the immunoprecipitated material and these are used to join sequences held in close proximity. The linker is biotinylated and contain MmeI restriction sites that allows for the extraction of short fragments of the material using a streptavidin bead pull-down. Paired-end tags can then be identified by paired-end sequencing.

Figure 1. Schematic Representation of ChIA-PET Sequencing

Features of CHIA-PET Sequencing

1. Remote chromatin interaction data associated with a specific protein can be generated for the purpose of studying the spatial structure of the 3D genome in which this protein is involved
2. Reproducible chromatin shearing with a tight size distribution is required to minimize the capture of unspecific interactions
3. The generation of a comprehensive 3D interaction map involving the proteins of interest depends on the efficient enrichment of low as well as high affinity sites and therefore requires good epitope preservation during chromatin shearing

Advantages of Choosing Creative BioMart

1. One-stop solutions: we provide one-stop services for customers with different needs, including experimental design, sequencing strategy design, and subsequent customized bioinformatics analysis.
2. High-quality data: our experimental procedures ensure good epitope preservation to allow comprehensive enrichment of sequences bound by the protein of interest and to reduce the pull-down of nonspecific interactions.
3. Advanced analytical capabilities: with our professional bioinformatics analysis tools of CHIA-PET, our skilled bioinformatics specialists work closely with customers to provide both standard data analyses and customized bioinformatics analyses.

Workflow of CHIA-PET Sequencing at Creative BioMart

Figure 2. Workflow of CHIA-PET Sequencing Service at Creative BioMart

Based on the commitment of prompt communication and on-time reporting, our staffs will ensure a high-efficiency service to meet the strict project timelines. If you have any special questions or requirements, please feel free to contact us.

Reference
1. Davies J O J.; et al. How best to identify chromosomal interactions: a comparison of approaches. Nature Methods. 2017, 14(2): 125-134.