Histone Deacetylase Assays

The removal of the acetyl group by histone deacetylases (HDACs) could result in a more tightly compacted, heterochromatin conformation, thus negatively regulating gene expression. Aberrant HDAC activity often correlates with cancer and neurological disorders, therefore, HDAC is widely accepted as an attractive drug target. HDAC inhibitors are already known potential anticancer agents and several HDAC inhibitors have received FDA approval for the treatment of human diseases. Cell-based detection of the activity and inhibition of HDACs is important for clarifying mechanisms of epigenetic regulation of gene activation and silencing, and also beneficial to cancer diagnostics and therapeutics.

Except for Western blot assay, Creative BioMart also performs HDAC-Glo Class I/II cell based assay to rapidly and sensitively evaluate the potency of HDAC inhibitors and selectively profile HDAC inhibitors. The HDAC-Glo I/II assays are highly sensitive, allowing you to obtain a dynamic range of 10- to 100-fold higher than fluorescence methods. HDACs Class I/II from cells, extracts or purified enzyme sources are available for HDAC assay. A variety of cell lines of interest can also be customized and purchased at Creative BioMart.

Procedure of Histone Deacetylase Assays at Creative BioMart

Cells are primarily seeded into each well of the assay plate and treated with various concentrations of tested compound for hours, followed by the addition of HDAC-Glo Class I/II detection reagent to each well of the plate. Subsequently, luminescence signals are recorded. The IC50 (half maximal inhibitory concentration) curve is plotted based on a sigmoidal dose-response equation and the IC50 value is finally calculated.

Schematic procedure of histone deacetylase assay

Figure 1. Schematic procedure of histone deacetylase assay

HDAC assay services supplied by Creative BioMart are suitable for a variety of different cell lines. The skilled team at Creative BioMart guides you to choose the most suitable tools and reagents for your experiments to avoid possible short comes and maximize the resulted efficiency of every step. At the same time, we can also provide tailored services according to customer needs. With the support of Creative BioMart, your epigenetic drug research is on the accelerated track of success. For more detailed information, please don’t hesitate to contact us.

1. Ropero, S.; Esteller, M. The role of histone deacetylases (HDACs) in human cancer. Mol Oncol. 2007, 1(1): 19-25.

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