Given a large amount of time and cost spent on chromatin immunoprecipitation (ChIP) and its downstream applications, we believe that the last thing you want to worry about is the quality of the antibodies used, and antibodies that are misbehaving can cause a lot of trouble in your analysis.

The success of ChIP and its subsequent applications such as ChIP-Seq depends to a large extent on the quality of the antibody, and for antibodies that work in ChIP, they must have high titers, high affinity, and sensitivity. In particular, antibodies for ChIP-Seq needs to recognize the correct target protein in all sequence contexts across the entire genome. Therefore, careful validation of antibodies utilized in ChIP experiments is important for the integrity and success of the entire ChIP study. Creative BioMart specializes in producing high-quality ChIP antibodies for histones, histone modifications, and other chromatin proteins, and verifying whether they are available for ChIP and its downstream applications. Creative BioMart guarantees that the antibodies used in our ChIP-based services are internally validated. In addition, our technical team provides ChIP antibody validation service, and we will determine whether your antibody is suitable for ChIP, ChIP-Seq, ChIP-qPCR or ChIP-on-chip.

General Workflow of ChIP Antibody Validation at Creative BioMart

1) We determine the specificity of antibodies by using complementary methods such as Western blot, immunofluorescence, peptide arrays, and peptide competition assays, especially important for histone modifications;

2) The most specific antibodies are tested in ChIP using an optimized antibody screening method;

3) After the ChIP quality control (QC), antibodies are evaluated using a small-scale ChIP-Seq assay;

4) Evaluating the antibody in a standardized ChIP-Seq analysis method based on ENCODE and modENCODE recommendations and using proprietary bioinformatics software settings.

Service Categories of ChIP Antibody Validation

1. ELISA
2. Western Blotting
3. Immunohistochemistry (IHC)
4. Immunofluorescence (ICC/IF)
5. Immunoprecipitation (IP)
6. Antibody Labeling
7. ChIP & Small-Scale ChIP-Seq

Advantages of Choosing Creative BioMart

1. One-stop service: customers only need to provide samples, and Creative BioMart completes full ChIP antibody validation. Project consultation is available free of charge if needed.
2. Reliable data quality: we have strict QC for each step of ChIP antibody validation experiment to ensure that customers receive high-quality data.
3. Professional data analysis: we have a professional bioinformatics analysis team to provide data analysis on request.

If you have any additional requirements or questions about our ChIP antibody validation service, please don’t hesitate to contact us for more information. Creative BioMart is also a leading provider of kits, reagents, and antibodies for the study of chromatin biology and epigenetics.

Reference
1. Wardle F C.; Tan H. A ChIP on the shoulder? Chromatin immunoprecipitation and validation strategies for ChIP antibodies. F1000research. 2015, 4: 235.