CAD
-
Official Full Name
carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
-
Overview
The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. -
Synonyms
CAD; carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase; CAD protein; CAD trifunctional protein; multifunctional protein CAD;
- Recombinant Proteins
- Protein Pre-coupled Magnetic Beads
- Human
- Mouse
- Zebrafish
- E.coli
- HEK293
- Mammalian Cell
- His
- His (Fc)
- Avi
| Species | Cat.# | Product name | Source (Host) | Tag | Protein Length | Price |
|---|---|---|---|---|---|---|
| Human | CAD-49H | Recombinant Human CAD protein, His-tagged | E.coli | His | Arg87~Lys323 | |
| Human | CAD-10649H | Recombinant Human CAD, His-tagged | E.coli | His | C-term-350a.a. | |
| Mouse | CAD-2634M | Recombinant Mouse CAD Protein | Mammalian Cell | His |
|
|
| Mouse | CAD-1179M-B | Recombinant Mouse CAD Protein Pre-coupled Magnetic Beads | HEK293 |
|
||
| Mouse | CAD-1179M | Recombinant Mouse CAD Protein, His (Fc)-Avi-tagged | HEK293 | His (Fc)-Avi |
|
|
| Zebrafish | CAD-1966Z | Recombinant Zebrafish CAD | Mammalian Cell | His |
|
- Involved Pathway
- Protein Function
- Interacting Protein
- CAD Related Articles
CAD involved in several pathways and played different roles in them. We selected most pathways CAD participated on our site, such as Pyrimidine metabolism, Alanine, aspartate and glutamate metabolism, Metabolic pathways, which may be useful for your reference. Also, other proteins which involved in the same pathway with CAD were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Pyrimidine metabolism | NME7;UPB1;NT5C;POLR2J;TK2;CANT1A;POLR1D;NT5E;POLR2G |
| Alanine, aspartate and glutamate metabolism | ADSSL;GFPT2;GAD1B;DDO;ABAT;GLULB;GPT2;ADSSL1;ASL2 |
| Metabolic pathways | PIP5K1BB;GALNTL1;ITGB1BP3;BCO1;TWISTNB;ADH1B;ACAA1A;GGPS1;POLR3GL |
CAD has several biochemical functions, for example, ATP binding, aspartate binding, aspartate carbamoyltransferase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by CAD itself. We selected most functions CAD had, and list some proteins which have the same functions with CAD. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| ATP binding | LACE1;PIP4K2B;CLPX;PAK3;SGK2B;ABCC10;UBE2L3;ERCC6L;UBE2Q1 |
| aspartate binding | |
| aspartate carbamoyltransferase activity | |
| carbamoyl-phosphate synthase (ammonia) activity | CPS1;CAD |
| carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity | CAD;CPS1 |
| dihydroorotase activity | |
| enzyme binding | HIST1H2AG;LUC7L2;CA;TSPAN5;H2AFX;HMGA1;NLRP1;GOT2;HIST1H2AI |
| identical protein binding | IAPP;CLDN4;GCG;PKD2L1;MPST;DYNLT1F;MBIP;SEPT7;CLDN23 |
| protein kinase activity | PRKD2;CLK2B;GUCY2F;PRKDC;NEK2;TNIK;MAP4K6;CAMK1GB;GRK1 |
| zinc ion binding | MKRN1;ZNF257;RPH3A;ADAM8;ZC3HC1;TOP3A;ZFML;ADAMTS16;RBM14A |
CAD has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with CAD here. Most of them are supplied by our site. Hope this information will be useful for your research of CAD.
ILK; NFKB2; NFKBIE; MAP3K7; TNFRSF1A; TNFRSF1B; YWHAZ; NFKB2; RELB; RELA; TRAF2; MAP3K1; RIPK1; TRADD; SMAD2; MAP3K14; MAP3K3; TAB2
- Q&As
- Reviews
Q&As (5)
Ask a questionCAD is allosterically regulated by UTP, which inhibits its activity, and ATP, which activates it. These nucleotides bind to specific sites on CAD and modulate its function, helping to control pyrimidine biosynthesis.
CAD is a multi-domain enzyme that catalyzes three sequential reactions in de novo pyrimidine biosynthesis: carbamoyl phosphate synthesis, aspartate carbamoylation, and dihydroorotate formation.
CAD helps maintain nucleotide balance by ensuring an adequate supply of pyrimidine nucleotides. When the cell needs more pyrimidines, CAD activity increases, and when there is an excess, CAD is allosterically inhibited.
Researchers use techniques like X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, and enzyme assays to study CAD's structure and function. These methods provide insights into its bio-molecular chemistry.
CAD is allosterically regulated by UTP and ATP. UTP negatively regulates CAD, while ATP positively regulates it. UTP binds to an allosteric site, promoting the formation of the less active CAD tetramer, whereas ATP stabilizes the more active CAD dimer.
Customer Reviews (3)
Write a reviewProduct arrived quickly and as described.
This product works wonders.
Quick shipping and well-packaged.
Ask a Question for All CAD Products
Required fields are marked with *
My Review for All CAD Products
Required fields are marked with *
