Nutritive Index Test Service

The Role of Nutritional Testing in Food Labeling and Product Development

The nutritive index of a food is an indicator of its total nutritional level, reflecting the presence and quantities of various essential nutrients such as proteins, fats, carbohydrates, vitamins, minerals, and water. Many countries have recognized the importance of nutritive index testing and have included it as a mandatory test item for food products. This ensures that consumers have access to reliable information about the nutritional content of the foods they consume.

Components Analyzed

The Nutritive Index Test Service typically involves the analysis of several key components:

• Proteins : Measured to determine their content and quality, as proteins are crucial for growth, repair, and maintenance of body tissues.
• Fats : Including saturated, unsaturated, and trans fats, as well as specific fatty acids like omega-3 and omega-6. Fats are essential for energy, vitamin absorption, and maintaining body temperature.
• Carbohydrates : Including total sugars, reducing sugars, starch, and dietary fiber. Carbohydrates are the primary source of energy for the body.
• Vitamins and Minerals : Essential micronutrients that play vital roles in various physiological functions, such as immune support, bone health, and metabolic processes.
• Water Content : Important for determining the moisture level in foods, which can affect their quality, shelf life, and sensory properties

Creative BioMart offers comprehensive Food Nutritive Index Testing Services in full compliance with the standards set by the Association of Official Analytical Chemists (AOAC) and the National Standard Method (GB). To ensure accurate and reliable results, we employ a wide range of advanced analytical techniques, including HPLC, GC, LC, GC-MS/MS, LC-MS/MS, amino acid analyzer, moisture analyzer, etc.

Our Nutritive Index Test Service Offerings

Service Procedure

Service Details

Category	Test Items	Analytical Methods
Vitamins	A, B1, B2, B6, C, D, E, K1, Niacin, Pantothenic acid	HPLC
Fatty Acids	Saturated, Unsaturated, Trans, Omega-3s (DHA, EPA), Linoleic, Oleic, etc.	GC, GC-MS/MS
Proteins	α-Lactalbumin, β-Lactoglobulin, Casein, Lactoferrin	LC, LC-MS/MS
Amino Acids	20+ essential and non-essential amino acids	Amino Acid Analyzer, HPLC, LC-MS/MS
Carbohydrates	Total sugar, Sucrose, Starch, Reducing sugar, Dietary fiber, Crude fiber	GC, HPLC, GC-MS/MS
Water	Moisture content	Moisture Analyzer
Other Components	Taurine, Lutein, β-Carotene, Choline	HPLC, Biochemical Methods

Advantages of Our Nutritive Index Test Service

Standards-Driven Testing : We follow AOAC and GB National Methods, ensuring internationally recognized accuracy and reliability.

Advanced Instrumentation : We use state-of-the-art platforms like LC-MS/MS, GC-MS/MS, HPLC, amino acid analyzers, and more for precise quantification.

One-Stop Nutritional Analysis : From macro-nutrients (protein, fat, carbs) to micro-nutrients (vitamins, amino acids), we offer full-spectrum nutritive profiling under one roof.

Rapid Turnaround Time : Because time is nutrition—our streamlined workflow delivers reliable results fast without compromising quality.

Customizable Test Packages : Pick and choose what you need. We offer flexible panels for specific nutrients or full-spectrum analysis.

Expert Support & Consultation : Our nutrition analysts and technical scientists are here to help interpret results and offer guidance on product development, labeling, and regulatory compliance.

Case Studies on Nutritional Profiling

* NOTE: We prioritize confidentiality to safeguard our clients’ technology and intellectual property. As an alternative, we present selected published research articles as representative case studies. For details on the assay services and products used in these studies, please refer to the relevant sections of the cited literature.

Case 1: Nutritional profiling and polysaccharide digestibility of Nymphaea nouchali as functional food

Hassan Rupom et al ., 2025. doi:10.1016/j.focha.2025.100990

This study evaluates the nutraceutical and pharmacological potential of the stem of Nymphaea nouchali (Waterlily) from Bangladesh as an affordable functional food. Proximate analysis revealed significant moisture, ash, fat, and fiber content, while mineral analysis showed notable sodium, potassium, calcium, and iron levels. High phenolic, flavonoid, and antioxidant contents were confirmed, alongside identification of bioactive compounds like gallic acid and quercetin. Acid digestibility tests indicated a high non-digestible polysaccharide fraction, suggesting benefits for gut health.

Figure 1. FTIR spectrum of polysaccharide extracted from SPN. (Hassan Rupom et al ., 2025)

Case 2: B vitamin profiling in supplements via single-wavelength HPLC

Vyas et al ., 2025. doi:10.5530/ijpi.20250223

This study developed and validated a simple, sensitive, and cost-effective HPLC method for the simultaneous detection of eight water-soluble B-complex vitamins (B1, B2, B3, B5, B6, B7, B9, B12) in food supplements. Using a C18 column and single-wavelength detection (210 nm), the method achieved excellent linearity (r² > 0.99), low LOD/LOQ, and consistent performance across samples. It overcomes common analytical challenges and is well-suited for routine quality control of various food matrices.

Figure 2. HPLC Chromatogram of vitamins (water soluble) in Formulated Food Supplement. B1 (RT 2.793 min) 2) B3 (RT 3.985 min) 3) B6 (RT 5.967 min) 4) B5 (RT 17.269 min) 5) B2 (RT 24.314 min). (Vyas et al ., 2025)

What Clients Say About Our Nutrition Testing Accuracy

"Creative BioMart’s nutrient analysis service has been a critical part of our product reformulation initiative. Their unsaturated fatty acid profiling helped us identify imbalances in our omega-3 to omega-6 ratio, which was key to optimizing our heart-health focused product line. The turnaround time was excellent, and their reporting format made it easy for both R&D and marketing to digest the results."

— Senior Product Developer | Functional Food Company

"We needed a full-spectrum nutritive analysis—including vitamins, amino acids, and moisture content—for our latest infant formula formulation. Creative BioMart delivered results that were not only highly accurate but also formatted to meet both domestic GB standards and overseas regulatory expectations. Their scientific team even helped us interpret the data for nutritional labeling. A seamless experience from start to finish."

— Regulatory Affairs Manager | Infant Nutrition Manufacturer

"As part of our ingredient quality assurance program, we’ve relied on Creative BioMart for carbohydrate and dietary fiber testing in multiple botanical extracts. Their use of GC-MS/MS provided the resolution we needed to distinguish complex polysaccharides from residual sugars. Their technical depth and responsiveness make them a preferred partner in our supply chain QC process."

— Director of Quality Control | Herbal Supplement Company

"Creative BioMart supported our clean-label initiative by helping us verify protein content and amino acid composition in our plant-based protein blends. Their amino acid analysis was spot on and matched our internal formulation targets. With their help, we were able to strengthen our label claims and pass third-party audits with confidence."

— Head of R&D | Plant-Based Food Startup

FAQs on Our Nutritive Index Test Service

• Q: What kind of food products can be tested?

  A: We test everything from raw agricultural products to processed foods, functional beverages, supplements, infant formula, pet food, and more.

• Q: Are your results acceptable for regulatory or labeling purposes?

  A: Yes! All tests are performed using AOAC and GB-approved methods, making our reports suitable for regulatory submission, label claims, and R&D.

• Q: How long does testing take?

  A: Turnaround time typically ranges from 5 to 10 business days, depending on the number and type of components tested.

• Q: Do I need to send large samples?

  A: No. Most tests require only 50-200 grams depending on food type. We’ll guide you on sample size and packaging.

• Q: Can I request specific nutrients to be tested only?

  A: Absolutely! You can choose targeted nutrient panels such as just vitamins, just fats, or even just amino acids.

• Q: Is interpretation of the data included?

  A: Yes! Our reports come with clear explanations and optional consultation to help you apply the results to product development or labeling.

References:

1. Hassan Rupom M, Bashera MA, Jahan F, et al . Nutritional profiling and crude polysaccharide extraction from Nymphaea nouchali to evaluate the acid digestibility as prospective source of functional food. Food Chemistry Advances . 2025;7:100990. doi:10.1016/j.focha.2025.100990
2. Vyas T, Shah U, Patel J, Tiwari M. Analytical method development and validation for the simultaneous quantification of water-soluble vitamins (B complex) at single wavelength by HPLC in food supplement. Int J Pharm Investigation . 2025;15(3):909-916. doi:10.5530/ijpi.20250223