Principle & Protocol

Guide for Mass Spectrometry Identification of Samples Separated by Liquid Chromatography

Guide for Mass Spectrometry Identification of Samples Separated by Liquid Chromatography

This guideline aims to explain the method of separating peptide targets using a liquid chromatography column after pancreatic enzyme hydrolysis, and then detecting them through MALDI-TOF-TOF mass spectrometry.

Protocol of Mass Spectrometry Identification of Gel Separated Samples

Protocol of Mass Spectrometry Identification of Gel Separated Samples

The purpose of this manual is to help scientific researchers understand the mass spectrometry method, master the basic principle of matrix assisted laser desorption ionization time-of-flight mass spectrometry and the mass spectrometry identification method of gel separation samples, and finally be able to use GPS Explorer software to analyze the mass spectrometry data.

Guide for Mass Spectrometric Identification of Phosphorylated Peptides and Phosphorylated Sites

Guide for Mass Spectrometric Identification of Phosphorylated Peptides and Phosphorylated Sites

This experimental manual aims to help scientific researchers master the basic principles and main steps of mass spectrometry identification of phosphorylated peptides and phosphorylated sites, and understand the methods and applications of mass spectrometry identification of phosphorylated peptides and phosphorylated sites.

Guide for Enrichment of Phosphorylated Peptides by Strong Cation Exchange Chromatography

Guide for Enrichment of Phosphorylated Peptides by Strong Cation Exchange Chromatography

This lab manual is designed to help researchers master the basic principles and main steps of strong cation exchange chromatography for the enrichment of phosphorylated proteins and understand the methods and applications of strong cation exchange chromatography for the detection of phosphorylated proteins.

Protocol of Immobilized Metal Affinity Chromatography

Protocol of Immobilized Metal Affinity Chromatography

This lab manual is designed to help researchers master the basic principles and main steps of enrichment of phosphorylated peptides by solid-phase metal ion affinity chromatography and understand the methods and applications of enrichment of phosphorylated peptides by solid-phase metal ion affinity chromatography.

Protocol of Affinity Enrichment of Phosphorylated Proteins

Protocol of Affinity Enrichment of Phosphorylated Proteins

This experimental guide aims to help scientific researchers master the basic principle and main steps of affinity enrichment method for detecting protein phosphorylation level, and understand the application of affinity enrichment method for detecting phosphorylated protein.

Protocol of the Phosphorylated Antibody Detection Method

Protocol of the Phosphorylated Antibody Detection Method

This experimental manual is designed to help scientific researchers master the basic principles and main steps of western blot detection of protein phosphorylation level, and understand the application of phosphorylation antibody detection methods.

Principle and Protocol of the PRO-Q Diamond Staining

Principle and Protocol of the PRO-Q Diamond Staining

The purpose of this experiment manual is to help scientific researchers master the basic principles and main steps of Pro-Q Diamond fluorescent dye to detect phosphorylated protein and SYPRO fluorescent dye to detect total protein, and understand the application of Pro-Q Diamond fluorescent dye to detect phosphorylated protein and SYPRO fluorescent dye to detect total protein.

Protocol of the Detection of ³²P Radioactive Markers

Protocol of the Detection of ³²P Radioactive Markers

The purpose of this lab manual is to help researchers master the basic principles and main steps of 32P radiolabeling detection of phosphorylated proteins and understand the application of 32P radiolabeling detection of phosphorylated proteins method.

Principle and Protocol of Isotope Labeling

Principle and Protocol of Isotope Labeling

The purpose of this experiment guide is to help researchers master the basic principles of iTRAQ technology and understand the experimental process and precautions of iTRAQ.

Principle and Protocol of Fluorescence Labeled Differential Analysis Gel Electrophoresis

Principle and Protocol of Fluorescence Labeled Differential Analysis Gel Electrophoresis

The purpose of this experiment manual is to help researchers master the basic principles and main steps of using two-dimensional electrophoresis combined with fluorescent dye labeling to analyze differentially expressed proteins and the method of using DeCyder software to analyze differentially expressed proteins.

Guide of Analysis of Differentially Expressed Proteins by Two-dimensional Electrophoresis

Guide of Analysis of Differentially Expressed Proteins by Two-dimensional Electrophoresis

This experimental manual focuses on the main methods of two-dimensional electrophoresis, the analysis methods of differential proteins and the optimization of parameters.

Guide of PCR-assisted Analysis of Protein-DNA Sequence Specificity

Guide of PCR-assisted Analysis of Protein-DNA Sequence Specificity

The main purpose of this experimental manual is to help scientific researchers understand the principle of PCR-assisted protein-DNA sequence specificity analysis and master the main operating steps and precautions of PCR-assisted protein-DNA sequence specificity analysis.

Principle and Protocol of Yeast One-hybrid Analysis

Principle and Protocol of Yeast One-hybrid Analysis

The purpose of this experiment manual is to help scientific researchers understand the basic principle and application of yeast single hybridization, master the main steps and precautions of yeast single hybridization, learn the production and transformation of yeast receptive state, and the construction and screening of gene library.

Guide of Analysis of Transient Cell Interaction

Guide of Analysis of Transient Cell Interaction

The purpose of this experiment manual is to help scientific researchers understand the principle of cell transient interaction analysis and master the main operating steps and precautions of cell transient interaction analysis.

Principle and Protocol of ChIP

Principle and Protocol of ChIP

The purpose of this lab manual is to help researchers master the screening of DNA sequences interacting with target proteins (regulators), to obtain information on protein-DNA interactions, and to master the principles, applications, and major steps of ChIP.

Principle and Protocol of EMSA

Principle and Protocol of EMSA

The purpose of this laboratory manual is to help researchers understand the principles of electrophoretic mobility analysis techniques and to master the main operational steps and precautions for gel migration.

Guide of Analysis of Promoter Regulatory Elements Based on Plant CARE Prediction Method

Guide of Analysis of Promoter Regulatory Elements Based on Plant CARE Prediction Method

The purpose of this experiment manual is to help scientific researchers master the PlantCARE database software of plant cis-acting elements, and analyze the promoter regulatory elements through the PlantCARE online prediction method.

Principle and Protocol of Pull-down Technology

Principle and Protocol of Pull-down Technology

The purpose of this experiment manual is to help scientific researchers learn the experimental principle of pull-down technology to identify protein interaction, and be familiar with the operation process and precautions of MBP pulldown technology.

Guide of Protein Subcellular Co-localization

Guide of Protein Subcellular Co-localization

The purpose of this experiment manual is to help scientific researchers master the preparation of Arabidopsis protoplasts, learn the technology of detecting protein-protein interaction using protein subcellular co-localization method, and be familiar with the operation process and precautions of co-transfection technology.

Principle and Protocol of Co-IP

Principle and Protocol of Co-IP

The laboratory manual is designed to help researchers master the principles of immunoprecipitation and its applications, learn to apply immunoprecipitation methods to detect protein interactions, and become familiar with the operational procedures and precautions for immunoprecipitation techniques.

Principle and Protocol of Yeast Two-hybrid System

Principle and Protocol of Yeast Two-hybrid System

The purpose of this experiment manual is to help scientific researchers master the principle and application of the yeast two-hybrid Sos recruited system, learn to use the co-transformation method to screen the interacting proteins and be familiar with the operation process and precautions of the yeast two-hybrid system for library screening.

Principle and Protocol of Yeast Two Hybrid System

Principle and Protocol of Yeast Two Hybrid System

The purpose of this experimental protocol is to help researchers to grasp the principle and application of the classic yeast two hybrid system, learn the methods of preparation and transformation of yeast cell competent state, and be familiar with the operation process and experimental precautions of yeast two hybrid system for detecting protein interaction.

Principle and Protocol of Expression and Purification of MBP Fusion Protein

Principle and Protocol of Expression and Purification of MBP Fusion Protein

The purpose of this experimental protocol is to help researchers to understand the basic principle of purification of maltose binding protein (MBP) fusion protein. Master the expression and purification methods and main operation steps of MBP fusion protein.

Principle and Protocol of Expression and Purification of His Fusion Protein

Principle and Protocol of Expression and Purification of His Fusion Protein

The purpose of this experimental protocol is to help researchers to understand the basic principle of fusion protein expression and purification. Master the expression and purification methods of histidine labeled fusion protein, as well as the main operation steps and precautions.

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