Membrane Proteins Expression and Purification

Membrane proteins play an essential role in many life activities of living organisms, such as cell proliferation and differentiation, energy conversion, signal transduction, and material transport. It is estimated that about 60% of drug targets are membrane proteins. Membrane proteins are important components of cell membranes and play an essential role in a variety of cellular functions such as substance transport, signal transduction, and intercellular recognition.

The human genome encodes about 25% of the total proteome of membrane proteins. These membrane proteins are associated with a variety of diseases such as cystic fibrosis, atherosclerosis, Parkinson's disease, and Alzheimer's disease. Thus, membrane proteins are important targets for numerous drug discovery and clinical therapeutic studies. Ion channels, transporter proteins, GPCRs, and kinases are the largest class of drug targets in drug discovery and development, and approximately 40% of approved antibody drugs exert their pharmacological effects by targeting GPCR complexes.

Membrane proteins limit their research progress due to low expression and difficulty in purification.

Creative Biomart has established a comprehensive membrane protein platform that provides enrichment and purification of membrane proteins, including Virus-like particles (VLPs) technology platform, detergent technology platform, Nanodisc technology platform, and liposome technology platform.

VLPs Technology Platform

VLP is a class of virus-like nanoscale particles structurally similar to real viruses but do not contain viral genes, providing a high degree of biosafety. In recent years, VLP technology has been widely used in vaccine development, drug carriers, gene therapy, and immunomodulation.

Detergent Technology Platform

Through the formation of detergent micelle on the cell membrane by detergent, the environment of the cell membrane is simulated to realize the expression and extraction of transmembrane proteins.

Nanodisc Technology Platform

This platform uses substances with both hydrophobic and hydrophilic properties as stabilizers. The hydrophobic surface facing the inner lipid layer integrates membrane proteins into Nanodisc, maintaining the natural spatial conformation and activity of membrane proteins. The hydrophilic surface facing outward enables Nanodisc to have high solubility and stability in aqueous solution.

Liposome Technology Platform

Using amphiphilic copolymers, target membrane proteins are extracted and separated from natural lipids without the use of detergents. Stable, highly active membrane proteins are obtained by liposome technology.

Creative Biomart has extensive experience in membrane protein isolation and purification, such as GPCRs, ion channels, transporters, membrane enzymes, and membrane fusion proteins.

Whether the target membrane protein is tagged, single or multi-pass transmembrane, single-protein, or large-protein complex, we can perform small- or industrial-scale protein isolation and purification. On this research platform, customers can always find the optimal solution.

Creative Biomart has accumulated lots of experience in membrane protein isolation and purification. Our experienced scientists, well-validated technology platforms, and state-of-the-art facilities guarantee our high-quality services. Our competitive prices and extensive expertise have earned us the trust of our collaborators. Contact us to find out how Creative Biomart could be of assistance.