Avi-tagged Proteins

The Avi tag is a 15 amino acid peptide tag with the sequence Gly-Leu-Asn-Asp-Ile-Phe-Glu-Ala-Gln-Lys-Ile-Glu-Trp-His-Glu. It is a popular fusion tag with powerful and versatile properties. Fused to the protein, the Avi tag provides a multi-functional system useful for many applications, including:

  • Expression
  • Imaging
  • Detection
  • Localization
  • Isolation
  • Immobilization

Biotinylation of the Avi tag is to covalently attach a biotin molecule to the lysine residue within the Avi-tag with E. coli biotin ligase (BirA). In vitro, BirA enzyme-labeled biotin efficiency is over 95%.

Advantages of Avi-tag Biotinylated Recombinant Proteins

  • Consistent labeling
  • Uniform protein orientation
  • Equivalent bioactivity

Avi-tag system protein can be simply and efficiently purified or adsorption fixed. Its number of applications in medical and industrial fields continues to increase. Creative BioMart provides a list of Avi-tagged proteins below. Please contact us for more information.

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