The Avi tag is a 15 amino acid peptide tag with the sequence Gly-Leu-Asn-Asp-Ile-Phe-Glu-Ala-Gln-Lys-Ile-Glu-Trp-His-Glu. It is a popular fusion tag with powerful and versatile properties. Fused to the protein, the Avi tag provides a multi-functional system useful for many applications, including:
Biotinylation of the Avi tag is to covalently attach a biotin molecule to the lysine residue within the Avi-tag with E. coli biotin ligase (BirA). In vitro, BirA enzyme-labeled biotin efficiency is over 95%.
Advantages of Avi-tag Biotinylated Recombinant Proteins
- Consistent labeling
- Uniform protein orientation
- Equivalent bioactivity
Avi-tag system protein can be simply and efficiently purified or adsorption fixed. Its number of applications in medical and industrial fields continues to increase. Creative BioMart provides a list of Avi-tagged proteins below. Please contact us for more information.
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