Active Recombinant Full Length Human BMX Protein, N-His-tagged

• Cat.No.: BMX-27HFL
• Product Overview: N-terminal 6Histagged, recombinant, full length, human Bmx, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: BMX (also called ETK, Bone marrow tyrosine kinase gene in chromosome X protein, Epithelial and endothelial tyrosine kinase) is one of the non-receptor tyrosine kinases, located intracellularly. It is a Src homolog and contains an SH3 and SH2 domain upstream from the tyrosine kinase domain. Unlike other members of the BTK family kinases, which are mostly hemopoietic cell-specific, BMX is preferentially expressed in epithelial and endothelial cells of large arteries. Inhibitors of BMX may be useful in the treatment of atherosclerosis and cancer.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.872mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 325U/mg, where one unit of Bmx, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 79 kDa
• AA Sequence: 1 MHHHHHHMDT KSILEELLLK RSQQKKKMSP NNYKERLFVL TKTNLSYYEY DKMKRGSRKG 61 SIEIKKIRCV EKVNLEEQTP VERQYPFQIV YKDGLLYVYA SNEESRSQWL KALQKEIRGN 121 PHLLVKYHSG FFVDGKFLCC QQSCKAAPGC TLWEAYANLH TAVNEEKHRV PTFPDRVLKI 181 PRAVPVLKMD APSSSTTLAQ YDNESKKNYG SQPPSSSTSL AQYDSNSKKI YGSQPNFNMQ 241 YIPREDFPDW WQVRKLKSSS SSEDVASSNQ KERNVNHTTS KISWEFPESS SSEEEENLDD 301 YDWFAGNISR SQSEQLLRQK GKEGAFMVRN SSQVGMYTVS LFSKAVNDKK GTVKHYHVHT 361 NAENKLYLAE NYCFDSIPKL IHYHQHNSAG MITRLRHPVS TKANKVPDSV SLGNGIWELK 421 REEITLLKEL GSGQFGVVQL GKWKGQYDVA VKMIKEGSMS EDEFFQEAQT MMKLSHPKLV 481 KFYGVCSKEY PIYIVTEYIS NGCLLNYLRS HGKGLEPSQL LEMCYDVCEG MAFLESHQFI 541 HRDLAARNCL VDRDLCVKVS DFGMTRYVLD DQYVSSVGTK FPVKWSAPEV FHYFKYSSKS 601 DVWAFGILMW EVFSLGKQPY DLYDNSQVVL KVSQGHRLYR PHLASDTIYQ IMYSCWHELP 661 EKRPTFQQLL SSIEPLREKD KH
• Purity: Purity 81.3% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: BMX BMX non-receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: BMX
• Synonyms: BMX; BMX non-receptor tyrosine kinase; ETK; PSCTK2; PSCTK3
• Gene ID: 660
• mRNA Refseq: NM_203281.3
• Protein Refseq: NP_975010.1
• MIM: 300101
• UniProt ID: P51813