Active Recombinant Human CES1 Protein

• Cat.No.: CES1-106H
• Product Overview: Recombinant human CES1 protein without tag was expressed in E. coli.

Specification

• Description: This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Bio-activity: CES Activity Data(Unless otherwise stated, all assays are carried out at 30 centigrade in 50 mM potassium phosphate buffer (pH 7.4)/acetonitrile, 98/2 (v/v))
  Substrate: 4-nitrophenyl butyrate
  Linear with time up to: 10 min(Determined at 90 μM substrate, using a protein concentration of 15 μg/ml)
  Linear with protein up to: 50 μg/ml(Determined at 90 μM substrate)
  Vmax: 511 nmol/min/mg protein
  Km: 152 μM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: Protein Concentration: 10 mg/ml
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
• Publication:
  Cellular Target Deconvolution of Small Molecules Using a Selection-Based Genetic Screening Platform (2022)

Gene Information

• Gene Name: CES1 carboxylesterase 1 [ Homo sapiens (human) ]
• Official Symbol: CES1
• Synonyms: CES1; carboxylesterase 1; CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1; liver carboxylesterase 1; acyl coenzyme A:cholesterol acyltransferase; brain carboxylesterase hBr1; carboxylesterase 1 (monocyte/macrophage serine esterase 1); carboxylesterase 2 (liver); cholesteryl ester hydrolase; cocaine carboxylesterase; egasyn; human monocyte/macrophage serine esterase 1; methylumbelliferyl-acetate deacetylase 1; monocyte/macrophage serine esterase; retinyl ester hydrolase; serine esterase 1; triacylglycerol hydrolase
• Gene ID: 1066
• mRNA Refseq: NM_001025194
• Protein Refseq: NP_001020365
• MIM: 114835
• UniProt ID: P23141

Reviews

03/29/2022

Reliable for lab work.

09/16/2021

Great value for quality.

09/03/2020

Good quality, great service.

Q&As

What is the primary function of the CES1 enzyme in the human body?

CES1 hydrolyzes ester and amide bonds in various compounds, including drugs, xenobiotics, and endogenous molecules.

In which tissues or organs is CES1 most abundantly expressed?

CES1 is most abundantly expressed in the liver, playing a significant role in first-pass metabolism.

How does CES1 contribute to drug metabolism?

It metabolizes numerous ester-containing drugs, affecting their pharmacokinetics and pharmacodynamics.

What is the significance of CES1 in the metabolism of prodrugs?

CES1 is crucial for the activation of certain prodrugs, converting them into their active pharmacological forms.

How can genetic variations in the CES1 gene affect drug response?

Genetic variations in CES1 can lead to inter-individual differences in drug metabolism, impacting efficacy and toxicity.

Are there known inhibitors or activators of CES1, and how might they influence its activity?

Certain compounds can inhibit or activate CES1, altering the metabolism of its substrates.

What role does CES1 play in lipid metabolism?

It participates in the hydrolysis of triglycerides and cholesterol esters, contributing to lipid homeostasis.