Recombinant Human GNL3L Protein (1-582 aa), His-SUMO-tagged

• Cat.No.: GNL3L-1121H
• Product Overview: Recombinant Human GNL3L Protein (1-582 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Protein Description: Full Length.

Specification

• Description: Stabilizes TERF1 telomeric association by preventing TERF1 recruitment by PML. Stabilizes TERF1 protein by preventing its ubiquitination and hence proteasomal degradation. Does so by interfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase. Required for cell proliferation. By stabilizing TRF1 protein during mitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2 protein by preventing its ubiquitination, and hence proteasomal degradation. By acting on MDM2, may affect TP53 activity. Required for normal processing of ribosomal pre-rRNA. Binds GTP.
• Source: E. coli
• Species: Human
• Tag: His&SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 81.6 kDa
• Protein length: 1-582 aa
• AA Sequence: MMKLRHKNKKPGEGSKGHKKISWPY PQPAKQNGKKATSKVPSAPHFVHPN DHANREAELKKKWVEEMREKQQAAR EQERQKRRTIESYCQDVLRRQEEFE HKEEVLQELNMFPQLDDEATRKAYY KEFRKVVEYSDVILEVLDARDPLGC RCFQMEEAVLRAQGNKKLVLVLNKI DLVPKEVVEKWLDYLRNELPTVAFK ASTQHQVKNLNRCSVPVDQASESLL KSKACFGAENLMRVLGNYCRLGEVR THIRVGVVGLPNVGKSSLINSLKRS RACSVGAVPGITKFMQEVYLDKFIR LLDAPGIVPGPNSEVGTILRNCVHV QKLADPVTPVETILQRCNLEEISNY YGVSGFQTTEHFLTAVAHRLGKKKK GGLYSQEQAAKAVLADWVSGKISFY IPPPATHTLPTHLSAEIVKEMTEVF DIEDTEQANEDTMECLATGESDELL GDTDPLEMEIKLLHSPMTKIADAIE NKTTVYKIGDLTGYCTNPNRHQMGW AKRNVDHRPKSNSMVDVCSVDRRSV LQRIMETDPLQQGQALASALKNKKK MQKRADKIASKLSDSMMSALDLSGN ADDGVGD
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: GNL3L guanine nucleotide binding protein-like 3 (nucleolar)-like [ Homo sapiens ]
• Official Symbol: GNL3L
• Synonyms: GNL3L; FLJ10613; novel GTPase; RP11-353K22.1
• Gene ID: 54552
• mRNA Refseq: NM_001184819
• Protein Refseq: NP_001171748
• UniProt ID: Q9NVN8