Recombinant Human EZH2 protein, His-tagged

• Cat.No.: EZH2-45H
• Product Overview: Recombinant Human EZH2 protein(NP_001190176.1)(Glu51~Phe285) was expressed in E. coli with a N-terminal His Tag.

Specification

• Description: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
• Molecular Mass: 28kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Glu51~Phe285
• AA Sequence: ERTEILNQEWKQRRIQPVHILTSVS SLRGTRECSVTSDLDFPTQVIPLKT LNAVASVPIMYSWSPLQQNFMVEDE TVLHNIPYMGDEVLDQDGTFIEELI KNYDGKVHGDRECGFINDEIFVELV NALGQYNDDDDDDDGDDPEEREEKQ KDLEDHRDDKESRPPRKFPSDKIFE AISSMFPDKGTAEELKEKYKELTEQ QLPGALPPECTPNIDGPNAKSVQRE QSLHSFHTLF
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)
• Notes: The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: EZH2
• Official Symbol: EZH2
• Synonyms: ENX-1; KMT6; KMT6A; Histone-lysine N-methyltransferase EZH2; Lysine N-methyltransferase 6
• Gene ID: 2146
• mRNA Refseq: NM_001203247.2
• Protein Refseq: NP_001190176.1
• MIM: 601573
• UniProt ID: Q15910

Reviews

10/17/2022

The versatility of the EZH2 protein is truly remarkable, as it can be employed across a wide range of applications in my research.

03/14/2018

In addition to its outstanding quality, the manufacturer of the EZH2 protein offers excellent technical support that can effectively address any challenges I may encounter.

02/27/2018

Their dedicated team of experts is readily available to provide guidance and assistance, ensuring a seamless integration of the EZH2 protein into my experimental setup.

Q&As

How is EZH2 overexpression associated with cancer progression?

EZH2 overexpression is associated with promoting tumor cell proliferation, invasion, and metastasis, leading to cancer progression.

Are there any non-oncological clinical applications of EZH2?

EZH2 has been implicated in various non-oncological conditions, including cardiovascular diseases and neurodegenerative disorders.

What are the potential side effects of EZH2 inhibitors in clinical use?

Potential side effects may include hematological toxicity, gastrointestinal issues, and liver enzyme elevation.

How can EZH2 be targeted for cancer therapy?

Small molecule inhibitors of EZH2 have been developed and are being investigated as potential cancer therapies.

Are there any prognostic implications of EZH2 expression in cancer patients?

High EZH2 expression has been associated with poor prognosis in several cancer types.