Recombinant Human Polo-like Kinase 4, GST-His

• Cat.No.: PLK4-705H
• Product Overview: Recombinant Human PLK4, full length, amino acids M1-H970, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9insect cells.MW = 138,568 Da.

Specification

• Cat. No.: PLK4-705H
• Description: This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle.
• Source: Baculovirus infected Sf9 cells.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: SAK was confirmed as human SAK by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
• Storage Buffer: 50 mM HEPES, pH 7.5; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
• Concentration: 0.050 μg/μl (Bradford method using BSA [Sigma, cat#A-7638, Lot 79H7641] as standard protein)
• Specific Activity: 6 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!
• Protein length: M1-H970

Gene Information

• Gene Name: PLK4 polo-like kinase 4 [ Homo sapiens ]
• Synonyms: PLK4; polo-like kinase 4; SAK; STK18; serine/threonine-protein kinase PLK4; PLK-4; Snk akin kinase; serine/threonine kinase 18; serine/threonine-protein kinase 18; serine/threonine protein kinase SAK; serine/threonine-protein kinase Sak; EC 2.7.11.21; polo-like kinase 4 (Drosophila)
• Gene ID: 10733
• mRNA Refseq: NM_001190799
• Protein Refseq: NP_001177728
• MIM: 605031
• UniProt ID: O00444
• Chromosome Location: 4q28
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; protein tyrosine kinase activity; transferase activity

Reviews

06/17/2023

With PLK4, the sensitivity of the experiment was significantly improved.

04/08/2022

Strong anti-pollution ability, not easy to pollute, to ensure the accuracy of the experiment.

11/07/2020

The method is very simple, and it is easy to obtain accurate experimental results.

Q&As

Is the interaction between PLK4 protein and other proteins?

There is an interaction between PLK4 protein and other proteins. For example, it interacts with certain proteins on spindle fibers to regulate chromosome segregation, and with other proteins in signal transduction pathways to regulate cell proliferation and differentiation.

What is the function of PLK4 protein in cell division?

PLK4 protein is mainly responsible for regulating the process of chromosome segregation and cytoplasmic division in cell division. It is associated with the assembly and function of spindle fibers, affecting the accuracy of chromosome segregation and cell division.

Which biological molecules can be used as molecular markers of PLK4 protein?

Certain biomolecules can be used as molecular markers of PLK4 protein, such as mRNA or protein of PLK4 protein detected in biological samples such as blood and urine. These markers can be used for early diagnosis of disease, prognostic judgment, or monitoring of treatment efficacy.

What is the current stage of research for the treatment of PLK4 protein?

Therapeutics targeting the PLK4 protein are still in the early stages of research. A number of small molecule inhibitors and antibody drugs have been developed and initially evaluated in laboratories and clinical trials. However, further research and clinical validation of these treatments are needed to determine their safety and efficacy.

What are the therapeutic strategies for PLK4 protein?

Therapeutic strategies for PLK4 protein include inhibition of its activity, regulation of its expression, and gene therapy. For example, the development of small molecule inhibitors or antibody drugs against the PLK4 protein to inhibit its aberrant activity in tumors, or the regulation of PLK4 protein expression through gene knockout or overexpression techniques.

Does abnormal PLK4 protein expression cause disease?

Studies have shown that the abnormal expression of PLK4 protein is associated with the occurrence of a variety of tumors, including but not limited to brain cancer, lung cancer, breast cancer, pancreatic cancer, etc. It may be related to aspects such as proliferation, metastasis, and drug resistance of tumor cells.