Active Recombinant Full Length Human CDK14 and CCNY co-expressed Protein, N-His-tagged

• Cat.No.: CDK14-CCNY-42HFL
• Product Overview: N-terminal 6His-tagged, recombinant, human CDK14 full length and N-terminal 6His-tagged, recombinant, human cyclin Y full length, co-expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: CDK14, aka PFTK1, is a member of the CDC2-related protein kinase family. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is CDK15.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.57mg/ml of enzyme in 50mM Tris/HCl pH7.5, 280mM NaCl, 260mM Imidazole, 0.09mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.9mM benzamidine, 0.2mM PMSF, 0.09% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 577U/mg, where one unit of CDK14/cyclin Y activity is defined as 1nmol phosphate incorporated into 200μM YRRAAVPPSPSLSRHSSPHQS(p)EDEEE per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: MW = 54kDa (CDK14) and 44kDa (cyclin Y).
• AA Sequence: CDK14 amino acid sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MHGYFGCNAA AEPGYSAFVG TPQICVTKMS 61 TRNCQGMDSV IKPLDTIPED KKVRVQRTQS TFDPFEKPAN QVKRVHSENN ACINFKTSST 121 GKESPKVRRH SSPSSPTSPK FGKADSYEKL EKLGEGSYAT VYKGKSKVNG KLVALKVIRL 181 QEEEGTPFTA IREASLLKGL KHANIVLLHD IIHTKETLTL VFEYVHTDLC QYMDKHPGGL 241 HPDNVKLFLF QLLRGLSYIH QRYILHRDLK PQNLLISDTG ELKLADFGLA RAKSVPSHTY 301 SNEVVTLWYR PPDVLLGSTE YSTCLDMWGV GCIFVEMIQG VAAFPGMKDI QDQLERIFLV 361 LGTPNEDTWP GVHSLPHFKP ERFTLYSSKN LRQAWNKLSY VNHAEDLASK LLQCSPKNRL 421 SAQAALSHEY FSDLPPRLWE LTDMSSIFTV PNVRLQPEAG ESMRAFGKNN SYGKSLSNSK 481 H
  cyclin Y amino acid sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRQMGNT TSCCVSSSPK LRRNAHSRLE 61 SYRPDTDLSR EDTGCNLQHI SDRENIDDLN MEFNPSDHPR ASTIFLSKSQ TDVREKRKSL 121 FINHHPPGQI ARKYSSCSTI FLDDSTVSQP NLKYTIKCVA LAIYYHIKNR DPDGRMLLDI 181 FDENLHPLSK SEVPPDYDKH NPEQKQIYRF VRTLFSAAQL TAECAIVTLV YLERLLTYAE 241 IDICPANWKR IVLGAILLAS KVWDDQAVWN VDYCQILKDI TVEDMNELER QFLELLQFNI 301 NVPSSVYAKY YFDLRSLAEA NNLSFPLEPL SRERAHKLEA ISRLCEDKYK DLRRSARKRS 361 ASADNLTLPR WSPAIIS
• Purity: Combined purity 71% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDK14 cyclin dependent kinase 14 [ Homo sapiens (human) ]&CCNY cyclin Y [ Homo sapiens (human) ]
• Official Symbol: CDK14 and CCNY
• Synonyms: CDK14 and CCNY; cyclin dependent kinase 14 and cyclin Y; PFTK1; PFTAIRE1 and CCNX; CFP1; CBCP1; C10orf9
• Gene ID: 5218 and 219771
• mRNA Refseq: NM_012395.3 and NM_145012.6
• Protein Refseq: NP_036527.1 and NP_659449.3
• MIM: 610679 and 612786
• UniProt ID: O94921 and Q8ND76