Active Recombinant Full Length Human CDK16 and CCNY co-expressed Protein, N-GST-tagged

• Cat.No.: CDK16-CCNY-43HFL
• Product Overview: N-terminal GST-tagged, recombinant, human CDK16 full length and Nterminal GST-tagged, recombinant, human cyclin Y full length co-expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: CDK16, cyclin dependent kinase 16, belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It plays a role in vesicle-mediated transport processes and exocytosis. It also plays a role in the regulation of insulin secretion in response to changes in blood glucose levels.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 2.72mg/ml of enzyme in 50mM Tris/HCl pH8.0, 4mM glutathione, 266mM NaCl, 0.09mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.9mM benzamidine, 0.2mM PMSF, 0.09% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 330U/mg, where one unit of CDK16/cyclin Y activity is defined as 1nmol phosphate incorporated into 125 μM PKSPKARKKL per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: MW = 83kDa (CDK16) and 67kDa (cyclin Y).
• AA Sequence: CDK16 amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFM DRMKKIKRQL 241 SMTLRGGRGI DKTNGAPEQI GLDESGGGGG SDPGEAPTRA APGELRSARG PLSSAPEIVH 301 EDLKMGSDGE SDQASATSSD EVQSPVRVRM RNHPPRKIST EDINKRLSLP ADIRLPEGYL 361 EKLTLNSPIF DKPLSRRLRR VSLSEIGFGK LETYIKLDKL GEGTYATVYK GKSKLTDNLV 421 ALKEIRLEHE EGAPCTAIRE VSLLKDLKHA NIVTLHDIIH TEKSLTLVFE YLDKDLKQYL 481 DDCGNIINMH NVKLFLFQLL RGLAYCHRQK VLHRDLKPQN LLINERGELK LADFGLARAK 541 SIPTKTYSNE VVTLWYRPPD ILLGSTDYST QIDMWGVGCI FYEMATGRPL FPGSTVEEQL 601 HFIFRILGTP TEETWPGILS NEEFKTYNYP KYRAEALLSH APRLDSDGAD LLTKLLQFEG 661 RNRISAEDAM KHPFFLSLGE RIHKLPDTTS IFALKEIQLQ KEASLRSSSM PDSGRPAFRV 721 VDTEF
  cyclin Y amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFK GLRRQMGNTT 241 SCCVSSSPKL RRNAHSRLES YRPDTDLSRE DTGCNLQHIS DRENIDDLNM EFNPSDHPRA 301 STIFLSKSQT DVREKRKSLF INHHPPGQIA RKYSSCSTIF LDDSTVSQPN LKYTIKCVAL 361 AIYYHIKNRD PDGRMLLDIF DENLHPLSKS EVPPDYDKHN PEQKQIYRFV RTLFSAAQLT 421 AECAIVTLVY LERLLTYAEI DICPANWKRI VLGAILLASK VWDDQAVWNV DYCQILKDIT 481 VEDMNELERQ FLELLQFNIN VPSSVYAKYY FDLRSLAEAN NLSFPLEPLS RERAHKLEAI 541 SRLCEDKYKD LRRSARKRSA SADNLTLPRW SPAIIS
• Purity: Purity 90% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDK16 cyclin dependent kinase 16 [ Homo sapiens (human) ]&CCNY cyclin Y [ Homo sapiens (human) ]
• Official Symbol: CDK16 and CCNY
• Synonyms: CDK16 and CCNY; cyclin dependent kinase 16 and cyclin Y; PCTK1; PCTAIRE; PCTAIRE1; PCTGAIRE and CCNX; CFP1; CBCP1; C10orf9
• Gene ID: 5127 and 219771
• mRNA Refseq: NM_006201.5 and NM_145012.6
• Protein Refseq: NP_006192.1 and NP_659449.3
• MIM: 311550 and 612786
• UniProt ID: Q00536 and Q8ND76