Active Recombinant Full Length Human CDK17 and CCNY co-expressed Protein, N-GST-tagged

• Cat.No.: CDK17-CCNY-44HFL
• Product Overview: N-terminal GST-tagged, recombinant, human CDK17 full length and Nterminal GST-tagged, recombinant, human cyclin Y full length co-expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: CDK17, cyclin dependent kinase 17, belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in terminally differentiated neurons and has a Ser/Thr-phosphorylating activity for histone H1 (By similarity).
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 1.11mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 489U/mg, where one unit of CDK17/cyclin Y activity is defined as 1nmol phosphate incorporated into 125 μM PKSPKARKKL per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: MW = 87kDa (CDK17) and 67kDa (cyclin Y).
• AA Sequence: CDK17 amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MKKFKRRLSL 241 TLRGSQTIDE SLSELAEQMT IEENSSKDNE PIVKNGRPPT SHSMHSFLHQ YTGSFKKPPL 301 RRPHSVIGGS LGSFMAMPRN GSRLDIVHEN LKMGSDGESD QASGTSSDEV QSPTGVCLRN 361 RIHRRISMED LNKRLSLPAD IRIPDGYLEK LQINSPPFDQ PMSRRSRRAS LSEIGFGKME 421 TYIKLEKLGE GTYATVYKGR SKLTENLVAL KEIRLEHEEG APCTAIREVS LLKDLKHANI 481 VTLHDIVHTD KSLTLVFEYL DKDLKQYMDD CGNIMSMHNV KLFLYQILRG LAYCHRRKVL 541 HRDLKPQNLL INEKGELKLA DFGLARAKSV PTKTYSNEVV TLWYRPPDVL LGSSEYSTQI 601 DMWGVGCIFF EMASGRPLFP GSTVEDELHL IFRLLGTPSQ ETWPGISSNE EFKNYNFPKY 661 KPQPLINHAP RLDSEGIELI TKFLQYESKK RVSAEEAMKH VYFRSLGPRI HALPESVSIF 721 SLKEIQLQKD PGFRNSSYPE TGHGKNRRQS MLF
  cyclin Y amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFK GLRRQMGNTT 241 SCCVSSSPKL RRNAHSRLES YRPDTDLSRE DTGCNLQHIS DRENIDDLNM EFNPSDHPRA 301 STIFLSKSQT DVREKRKSLF INHHPPGQIA RKYSSCSTIF LDDSTVSQPN LKYTIKCVAL 361 AIYYHIKNRD PDGRMLLDIF DENLHPLSKS EVPPDYDKHN PEQKQIYRFV RTLFSAAQLT 421 AECAIVTLVY LERLLTYAEI DICPANWKRI VLGAILLASK VWDDQAVWNV DYCQILKDIT 481 VEDMNELERQ FLELLQFNIN VPSSVYAKYY FDLRSLAEAN NLSFPLEPLS RERAHKLEAI 541 SRLCEDKYKD LRRSARKRSA SADNLTLPRW SPAIIS
• Purity: Purity 80% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDK17 cyclin dependent kinase 17 [ Homo sapiens (human) ]&CCNY cyclin Y [ Homo sapiens (human) ]
• Official Symbol: CDK17 and CCNY
• Synonyms: CDK17 and CCNY; cyclin dependent kinase 17 and cyclin Y; PCTK2; PCTAIRE2 and CCNX; CFP1; CBCP1; C10orf9
• Gene ID: 5128 and 219771
• mRNA Refseq: NM_002595.5 and NM_145012.6
• Protein Refseq: NP_002586.2 and NP_659449.3
• MIM: 603440 and 612786
• UniProt ID: Q00537 and Q8ND76