Active Recombinant Full Length Human CDK18 and CCNY co-expressed Protein, N-GST-tagged

• Cat.No.: CDK18-CCNY-45HFL
• Product Overview: N-terminal GST-tagged, recombinant, human CDK18 full length and Nterminal GST-tagged, recombinant human cyclin Y full length expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: CDK18, cyclin dependent kinase 18, is predicted to enable ATP binding activity and to be involved in protein phosphorylation and regulation of transcription involved in G1/S transition of mitotic cell cycle. It may play a role in signal transduction cascades in terminally differentiated cells.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 3.85 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 144 U/mg, where one unit of CDK18/cyclin Y activity is defined as 1 nmol phosphate incorporated into 100 μM (YRRAAVPPSPSLSRHSSPHQS(p)EDEEE) per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: MW = 81 kDa (CDK18) and MW = 67 kDa (cyclin Y)
• AA Sequence: CDK18 amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKELT MIMNKMKNFK 241 RRFSLSVPRT ETIEESLAEF TEQFNQLHNR RNENLQLGPL GRDPPQECST FSPTDSGEEP 301 GQLSPGVQFQ RRQNQRRFSM EDVSKRLSLP MDIRLPQEFL QKLQMESPDL PKPLS RMSRR 361 ASLSDIGFGK LETYVKLDKL GEGTYATVFK GRSKLMENLV ALKEIRLEHE EGAPCTAIRE 421 VSLLKNLKHA NIVTLHDLIH TDRSLTLVFE YLDSDLKQYL DHCGNLMSMH NVKIFMFQLL 481 RGLAYCHHRK ILHRDLKPQN LLINERGELK LADFGLARAK SVPTKTYSNE VVTLWYRPPD 541 VLLGSTEYST PIDMWGVGCI HYEMATGRPL FPGSTVKEEL HLIFRLLGTP TEETWPGVTA 601 FSEFRTYSFP CYLPQPLINH APRLDTDGIH LLSSLLLYES KSRMSAEAAL SHSYFRSLGE 661 RVHQLEDTAS IFSLKEIQLQ KDPGYRGLAF QQPGRGKNRR QSIF
  cyclin Y amino acid sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFK GLRRQMGNTT 241 SCCVSSSPKL RRNAHSRLES YRPDTDLSRE DTGCNLQHIS DRENIDDLNM EFNPSDHPRA 301 STIFLSKSQT DVREKRKSLF INHHPPGQIA RKYSSCSTIF LDDSTVSQPN LKYTIKCVAL 361 AIYYHIKNRD PDGRMLLDIF DENLHPLSKS EVPPDYDKHN PEQKQIYRFV RTLFSAAQLT 421 AECAIVTLVY LERLLTYAEI DICPANWKRI VLGAILLASK VWDDQAVWNV DYCQILKDIT 481 VEDMNELERQ FLELLQFNIN VPSSVYAKYY FDLRSLAEAN NLSFPLEPLS RERAHKLEAI 541 SRLCEDKYKD LRRSARKRSA SADNLTLPRW SPAIIS
• Purity: Purity (CDK18 and cyclin Y combined) 72% by SDSPAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDK18 cyclin dependent kinase 18 [ Homo sapiens (human) ]&CCNY cyclin Y [ Homo sapiens (human) ]
• Official Symbol: CDK18 and CCNY
• Synonyms: CDK18 and CCNY; cyclin dependent kinase 18 and cyclin Y; PFTK1; PFTAIRE1 and CCNX; CFP1; CBCP1; C10orf9
• Gene ID: 5129 and 219771
• mRNA Refseq: NM_002596.4 and NM_145012.6
• Protein Refseq: NP_002587.2 and NP_659449.3
• MIM: 169190 and 612786
• UniProt ID: Q07002 and Q8ND76