Active Recombinant Full Length Human MST1 Protein, N-His-tagged

• Cat.No.: MST1-111HFL
• Product Overview: N-terminal 6Histagged, recombinant, full-length, human MST1, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTAagarose.

Specification

• Description: MST1 and MST2 contain a putative caspase cleavage site at the junction of the catalytic domain and the C-terminal region. MST is involved in a positive feedback loop in possible association with the SAPK and p38-MAPK pathways. MST1 has been shown activated in cellular apoptosis induced by a variety of stimuli. Inhibition of MST1 may inhibit Raf-1-mediated cell proliferation and result in apoptosis.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.607mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 645U/mg, where one unit of MST1, active activity is defined as 1nmol phosphate incorporated into 250μM (KKSRGDYMTMQIG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 57 kDa
• AA Sequence: 1 MHHHHHHEFM ETVQLRNPPR RQLKKLDEDS LTKQPEEVFD VLEKLGEGSY GSVYKAIHKE 61 TGQIVAIKQV PVESDLQEII KEISIMQQCD SPHVVKYYGS YFKNTDLWIV MEYCGAGSVS 121 DIIRLRNKTL TEDEIATILQ STLKGLEYLH FMRKIHRDIK AGNILLNTEG HAKLADFGVA 181 GQLTDTMAKR NTVIGTPFWM APEVIQEIGY NCVADIWSLG ITAIEMAEGK PPYADIHPMR 241 AIFMIPTNPP PTFRKPELWS DNFTDFVKQC LVKSPEQRAT ATQLLQHPFV RSAKGVSILR 301 DLINEAMDVK LKRQESQQRE VDQDDEENSE EDEMDSGTMV RAVGDEMGTV RVASTMTDGA 361 NTMIEHDDTL PSQLGTMVIN AEDEEEEGTM KRRDETMQPA KPSFLEYFEQ KEKENQINSF 421 GKSVPGPLKN SSDWKIPQDG DYEFLKSWTV EDLQKRLLAL DPMMEQEIEE IRQKYQSKRQ 481 PILDAIEAKK RRQQNF
• Purity: Purity 88.7% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MST1 macrophage stimulating 1 [ Homo sapiens (human) ]
• Official Symbol: MST1
• Synonyms: MST1; macrophage stimulating 1; MSP; HGFL; NF15S2; D3F15S2; DNF15S2
• Gene ID: 4485
• mRNA Refseq: NM_020998.4
• Protein Refseq: NP_066278.3
• MIM: 142408
• UniProt ID: P26927