Active Recombinant Full Length Human OSR1 and CAB39 co-expressed Protein, N-GST,N-GST-tagged
| Cat.No. : | OSR1-CAB39-118HFL |
| Product Overview : | N-terminal GST-tagged, recombinant, human OSR1 full length and Nterminal GST tagged, recombinant, human MO25α full length, co-expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose. Complex activated in vivo with recombinant WNK3. |
- Specification
- Gene Information
- Related Products
| Description : | The oxidative stress-responsive 1 (OSR1) kinase belongs to the mammalian STE20-like kinase family. OSR1 is activated by with no lysine [K] (WNKs) kinases, and then it phosphorylates cation-coupled Cl-cotransporters, regulating ion homeostasis and cell volume in mammalian cells. |
| Source : | Sf21 |
| Species : | Human |
| Tag : | GST,GST |
| Protein length : | Full Length |
| Form : | 0.64mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution. |
| Bio-activity : | 177U/mg, where one unit of OSR1 activity is defined as 1nmol phosphate incorporated into 125μM catchtide per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : | OSR1 MW = 86kDa; MO25α MW = 67kDa |
| AA Sequence : | 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFK GLRRRAQLVR 241 PQMSEDSSAL PWSINRDDYE LQEVIGSGAT AVVQAAYCAP KKEKVAIKRI NLEKCQTSMD 301 ELLKEIQAMS QCHHPNIVSY YTSFVVKDEL WLVMKLLSGG SVLDIIKHIV AKGEHKSGVL 361 DESTIATILR EVLEGLEYLH KNGQIHRDVK AGNILLGEDG SVQIADFGVS AFLATGGDIT 421 RNKVRKTFVG TPCWMAPEVM EQVRGYDFKA DIWSFGITAI ELATGAAPYH KYPPMKVLML 481 TLQNDPPSLE TGVQDKEMLK KYGKSFRKMI SLCLQKDPEK RPTAAELLRH KFFQKAKNKE 541 FLQEKTLQRA PTISERAKKV RRVPGSSGRL HKTEDGGWEW SDDEFDEESE EGKAAISQLR 601 SPRVKESISN SELFPTTDPV GTLLQVPEQI SAHLPQPAGQ IATQPTQVSL PPTAEPAKTA 661 QALSSGSGSQ ETKIPISLVL RLRNSKKELN DIRFEFTPGR DTAEGVSQEL ISAGLVDGRD 721 LVIVAANLQK IVEEPQSNRS VTFKLASGVE GSDIPDDGKL IGFAQLSIS |
| Purity : | Purity 97% (combined) by SDS-PAGE and Coomassie blue staining. |
| Applications : | Drug Discovery & Development |
| Storage : | 1 year at -70 centigrade. |
| Handling Advice : | Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : | On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
| Gene Name : | OSR1 odd-skipped related transcription factor 1 [ Homo sapiens (human) ] and CAB39 calcium binding protein 39 [ Homo sapiens (human) ] |
| Official Symbol : | OSR1 and CAB39 |
| Synonyms : | OSR1 and CAB39; odd-skipped related transcription factor 1 and calcium binding protein 39; ODD and MO25; CGI-66 |
| Gene ID : | 130497 and 51719 |
| mRNA Refseq : | NM_145260.3 and NM_016289.4 |
| Protein Refseq : | NP_660303.1 and NP_057373.1 |
| MIM : | 608891 and 612174 |
| UniProt ID : | Q8TAX0 and Q9Y376 |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket
