Active Recombinant Full Length Human PIK3CB and PIK3R1 Co-expressed Protein, N-His-tagged

• Cat.No.: PIK3CB-PIK3R1-135HFL
• Product Overview: Complex of N-terminal 6Histagged recombinant full-length human p110β and untagged, recombinant, full length, human p85α. Co-expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose.

Specification

• Description: Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.534mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 144U/mg, where one unit of PI3 Kinase (p110β) activity is defined as 1nmol phosphatidylinositol 3,4,5- trisphosphate formed per minute at 22 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: P110β MW = 124kDa, p85α MW = 83.7kDa
• AA Sequence: p110β amino acid sequence: 1 MHHHHHHEFM CFSFIMPPAM ADILDIWAVD SQIASDGSIP VDFLLPTGIY IQLEVPREAT 61 ISYIKQMLWK QVHNYPMFNL LMDIDSYMFA CVNQTAVYEE LEDETRRLCD VRPFLPVLKL 121 VTRSCDPGEK LDSKIGVLIG KGLHEFDSLK DPEVNEFRRK MRKFSEEKIL SLVGLSWMDW 181 LKQTYPPEHE PSIPENLEDK LYGGKLIVAV HFENCQDVFS FQVSPNMNPI KVNELAIQKR 241 LTIHGKEDEV SPYDYVLQVS GRVEYVFGDH PLIQFQYIRN CVMNRALPHF ILVECCKIKK 301 MYEQEMIAIE AAINRNSSNL PLPLPPKKTR IISHVWENNN PFQIVLVKGN KLNTEETVKV 361 HVRAGLFHGT ELLCKTIVSS EVSGKNDHIW NEPLEFDINI CDLPRMARLC FAVYAVLDKV 421 KTKKSTKTIN PSKYQTIRKA GKVHYPVAWV NTMVFDFKGQ LRTGDIILHS WSSFPDELEE 481 MLNPMGTVQT NPYTENATAL HVKFPENKKQ PYYYPPFDKI IEKAAEIASS DSANVSSRGG 541 KKFLPVLKEI LDRDPLSQLC ENEMDLIWTL RQDCREIFPQ SLPKLLLSIK WNKLEDVAQL 601 QALLQIWPKL PPREALELLD FNYPDQYVRE YAVGCLRQMS DEELSQYLLQ LVQVLKYEPF 661 LDCALSRFLL ERALGNRRIG QFLFWHLRSE VHIPAVSVQF GVILEAYCRG SVGHMKVLSK 721 QVEALNKLKT LNSLIKLNAV KLNRAKGKEA MHTCLKQSAY REALSDLQSP LNPCVILSEL 781 YVEKCKYMDS KMKPLWLVYN NKVFGEDSVG VIFKNGDDLR QDMLTLQMLR LMDLLWKEAG 841 LDLRMLPYGC LATGDRSGLI EVVSTSETIA DIQLNSSNVA AAAAFNKDAL LNWLKEYNSG 901 DDLDRAIEEF TLSCAGYCVA SYVLGIGDRH SDNIMVKKTG QLFHIDFGHI LGNFKSKFGI 961 KRERVPFILT YDFIHVIQQG KTGNTEKFGR FRQCCEDAYL ILRRHGNLFI TLFALMLTAG 1021 LPELTSVKDI QYLKDSLALG KSEEEALKQF KQKFDEALRE SWTTKVNWMA HTVRKDYRS
  p85α amino acid sequence:
  1 MSAEGYQYRA LYDYKKEREE DIDLHLGDIL TVNKGSLVAL GFSDGQEARP EEIGWLNGYN
  61 ETTGERGDFP GTYVEYIGRK KISPPTPKPR PPRPLPVAPG SSKTEADVEQ QALTLPDLAE
  121 QFAPPDIAPP LLIKLVEAIE KKGLECSTLY RTQSSSNLAE LRQLLDCDTP SVDLEMIDVH
  181 VLADAFKRYL LDLPNPVIPA AVYSEMISLA PEVQSSEEYI QLLKKLIRSP SIPHQYWLTL
  241 QYLLKHFFKL SQTSSKNLLN ARVLSEIFSP MLFRFSAASS DNTENLIKVI EILISTEWNE
  301 RQPAPALPPK PPKPTTVANN GMNNNMSLQD AEWYWGDISR EEVNEKLRDT ADGTFLVRDA
  361 STKMHGDYTL TLRKGGNNKL IKIFHRDGKY GFSDPLTFSS VVELINHYRN ESLAQYNPKL
  421 DVKLLYPVSK YQQDQVVKED NIEAVGKKLH EYNTQFQEKS REYDRLYEEY TRTSQEIQMK
  481 RTAIEAFNET IKIFEEQCQT QERYSKEYIE KFKREGNEKE IQRIMHNYDK LKSRISEIID
  541 SRRRLEEDLK KQAAEYREID KRMNSIKPDL IQLRKTRDQY LMWLTQKGVR QKKLNEWLGN
  601 ENTEDQYSLV EDDEDLPHHD EKTWNVGSSN RNKAENLLRG KRDGTFLVRE SSKQGCYACS
  661 VVVDGEVKHC VINKTATGYG FAEPYNLYSS LKELVLHYQH TSLVQHNDSL NVTLAYPVYA
  721 QQRR
• Purity: Purity 72.9% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PIK3CB phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta [ Homo sapiens (human) ] and PIK3R1 phosphoinositide-3-kinase regulatory subunit 1 [ Homo sapiens (human) ]
• Official Symbol: PIK3CB and PIK3R1
• Synonyms: PIK3CB and PIK3R1; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta and phosphoinositide-3-kinase regulatory subunit 1; PI3K; PIK3C1; P110BETA; PI3KBETA and p85; AGM7; GRB1; IMD36; p85alpha; p85-ALPHA
• Gene ID: 5291 and 5295
• mRNA Refseq: NM_006219.3 and NM_181504.4
• Protein Refseq: NP_006210.1 and NP_852556.2
• MIM: 602925 and 171833
• UniProt ID: P42338 and A0A2X0SFG1