Active Recombinant Full Length Human PIK3CD and PIK3R1 Co-expressed Protein, N-His-tagged

• Cat.No.: PIK3CD-PIK3R1-137HFL
• Product Overview: Complex of N-terminal 6Histagged recombinant, full-length, human p110δ and untagged, recombinant, full length, human p85α. Coexpressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose.

Specification

• Description: Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. See MIM 602838. The class I PI3Ks display a broad phosphoinositide lipid substrate specificity and include p110-alpha (MIM 171834), p110-beta (MIM 602925), and p110-gamma (MIM 601232). p110-alpha and p110-beta interact with SH2/SH3-domain-containing p85 adaptor proteins (see MIM 171833) and with GTP-bound Ras. GenBank NM_005026. The recombinant protein contains the amino acid substitution N253S with respect to this accession number. This conflict is reported in GenBank Y10055 and U57843. The residue coordinates in the native sequence are given / GenBank XM_043865.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 2.734mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 293U/mg, where one unit of PI 3-kinase (p110δ/p85α) activity is defined as 1nmol phosphatidylinositol 3,4,5- trisphosphate formed per minute at 22 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: P110δ MW = 121.6kDa, p85α MW = 83.7kDa
• AA Sequence: p110δ amino acid sequence: 1 MHHHHHHEFK GLRRRMPPGV DCPMEFWTKE ENQSVVVDFL LPTGVYLNFP VSRNANLSTI 61 KQLLWHRAQY EPLFHMLSGP EAYVFTCINQ TAEQQELEDE QRRLCDVQPF LPVLRLVARE 121 GDRVKKLINS QISLLIGKGL HEFDSLCDPE VNDFRAKMCQ FCEEAAARRQ QLGWEAWLQY 181 SFPLQLEPSA QTWGPGTLRL PNRALLVNVK FEGSEESFTF QVSTKDVPLA LMACALRKKA 241 TVFRQPLVEQ PEDYTLQVNG RHEYLYGSYP LCQFQYICSC LHSGLTPHLT MVHSSSILAM 301 RDEQSNPAPQ VQKPRAKPPP IPAKKPSSVS LWSLEQPFRI ELIQGSKVNA DERMKLVVQA 361 GLFHGNEMLC KTVSSSEVSV CSEPVWKQRL EFDINICDLP RMARLCFALY AVIEKAKKAR 421 STKKKSKKAD CPIAWANLML FDYKDQLKTG ERCLYMWPSV PDEKGELLNP TGTVRSNPNT 481 DSAAALLICL PEVAPHPVYY PALEKILELG RHSECVHVTE EEQLQLREIL ERRGSGELYE 541 HEKDLVWKLR HEVQEHFPEA LARLLLVTKW NKHEDVAQML YLLCSWPELP VLSALELLDF 601 SFPDCHVGSF AIKSLRKLTD DELFQYLLQL VQVLKYESYL DCELTKFLLD RALANRKIGH 661 FLFWHLRSEM HVPSVALRFG LILEAYCRGS THHMKVLMKQ GEALSKLKAL NDFVKLSSQK 721 TPKPQTKELM HLCMRQEAYL EALSHLQSPL DPSTLLAEVC VEQCTFMDSK MKPLWIMYSN 781 EEAGSGGSVG IIFKNGDDLR QDMLTLQMIQ LMDVLWKQEG LDLRMTPYGC LPTGDRTGLI 841 EVVLRSDTIA NIQLNKSNMA ATAAFNKDAL LNWLKSKNPG EALDRAIEEF TLSCAGYCVA 901 TYVLGIGDRH SDNIMIRESG QLFHIDFGHF LGNFKTKFGI NRERVPFILT YDFVHVIQQG 961 KTNNSEKFER FRGYCERAYT ILRRHGLLFL HLFALMRAAG LPELSCSKDI QYLKDSLALG 1021 KTEEEALKHF RVKFNEALRE SWKTKVNWLA HNVSKDNRQ
  p85α amino acid sequence:
  1 MSAEGYQYRA LYDYKKEREE DIDLHLGDIL TVNKGSLVAL GFSDGQEARP EEIGWLNGYN
  61 ETTGERGDFP GTYVEYIGRK KISPPTPKPR PPRPLPVAPG SSKTEADVEQ QALTLPDLAE
  121 QFAPPDIAPP LLIKLVEAIE KKGLECSTLY RTQSSSNLAE LRQLLDCDTP SVDLEMIDVH
  181 VLADAFKRYL LDLPNPVIPA AVYSEMISLA PEVQSSEEYI QLLKKLIRSP SIPHQYWLTL
  241 QYLLKHFFKL SQTSSKNLLN ARVLSEIFSP MLFRFSAASS DNTENLIKVI EILISTEWNE
  301 RQPAPALPPK PPKPTTVANN GMNNNMSLQD AEWYWGDISR EEVNEKLRDT ADGTFLVRDA
  361 STKMHGDYTL TLRKGGNNKL IKIFHRDGKY GFSDPLTFSS VVELINHYRN ESLAQYNPKL
  421 DVKLLYPVSK YQQDQVVKED NIEAVGKKLH EYNTQFQEKS REYDRLYEEY TRTSQEIQMK
  481 RTAIEAFNET IKIFEEQCQT QERYSKEYIE KFKREGNEKE IQRIMHNYDK LKSRISEIID
  541 SRRRLEEDLK KQAAEYREID KRMNSIKPDL IQLRKTRDQY LMWLTQKGVR QKKLNEWLGN
  601 ENTEDQYSLV EDDEDLPHHD EKTWNVGSSN RNKAENLLRG KRDGTFLVRE SSKQGCYACS
  661 VVVDGEVKHC VINKTATGYG FAEPYNLYSS LKELVLHYQH TSLVQHNDSL NVTLAYPVYA
  721 QQRR
  
• Purity: Purity 98.0% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PIK3CD phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta [ Homo sapiens (human) ] and PIK3R1 phosphoinositide-3-kinase regulatory subunit 1 [ Homo sapiens (human) ]
• Official Symbol: PIK3CD and PIK3R1
• Synonyms: PIK3CD and PIK3R1; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta and phosphoinositide-3-kinase regulatory subunit 1; APDS; PI3K; IMD14; p110D; IMD14A; IMD14B; ROCHIS; P110DELTA and p85; AGM7; GRB1; IMD36; p85alpha; p85-ALPHA
• Gene ID: 5293 and 5295
• mRNA Refseq: NM_005026.5 and NM_181504.4
• Protein Refseq: NP_005017.3 and NP_852556.2
• MIM: 602839 and 171833
• UniProt ID: O00329 and A0A2X0SFG1