Active Recombinant Full Length Human STK38 and MOB1B co-expressed Protein, N-GST,C-His,N-His-tagged
| Cat.No. : | STK38-MOB1B-113HFL |
| Product Overview : | N-terminal GST-tagged and C-terminal 6His-tagged, recombinant, human NDR1 full length and N-terminal 6His-tagged, recombinant, human MOBKL1A full length coexpressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose. Complex activated in vivo with recombinant MST2. |
- Specification
- Gene Information
- Related Products
| Description : | NDR1 is a member of the AGC serine/threonine kinase family of proteins. The kinase activity of this protein is regulated by autophosphorylation and phosphorylation by other upstream kinases. This protein has been shown to function in the cell cycle and apoptosis. This protein has also been found to regulate the protein stability and transcriptional activity of the MYC oncogene. Alternative splicing results in multiple transcript variants |
| Source : | Sf21 |
| Species : | Human |
| Tag : | GST,His,His |
| Protein length : | Full Length |
| Form : | 0.45mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution. |
| Bio-activity : | 1405U/mg, where one unit of NDR1 activity is defined as 1nmol phosphate incorporated into 150μM KKLRRTLSVA per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : | NDR1 MW = 83kDa, MOBKL1A MW = 30kDa. |
| AA Sequence : | 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFK GLRRQMAMTG 241 STPCSSMSNH TKERVTMTKV TLENFYSNLI AQHEEREMRQ KKLEKVMEEE GLKDEEKRLR 301 RSAHARKETE FLRLKRTRLG LEDFESLKVI GRGAFGEVRL VQKKDTGHVY AMKILRKADM 361 LEKEQVGHIR AERDILVEAD SLWVVKMFYS FQDKLNLYLI MEFLPGGDMM TLLMKKDTLT 421 EEETQFYIAE TVLAIDSIHQ LGFIHRDIKP DNLLLDSKGH VKLSDFGLCT GLKKAHRTEF 481 YRNLNHSLPS DFTFQNMNSK RKAETWKRNR RQLAFSTVGT PDYIAPEVFM QTGYNKLCDW 541 WSLGVIMYEM LIGYPPFCSE TPQETYKKVM NWKETLTFPP EVPISEKAKD LILRFCCEWE 601 HRIGAPGVEE IKSNSFFEGV DWEHIRERPA AISIEIKSID DTSNFDEFPE SDILKPTVAT 661 SNHPETDYKN KDWVFINYTY KRFEGLTARG AIPSYMKAAK HHHHHH |
| Purity : | Purity 97% (combined) by SDS-PAGE and Coomassie blue staining. |
| Applications : | Drug Discovery & Development |
| Storage : | 1 year at -70 centigrade. |
| Handling Advice : | Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : | On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
| Gene Name : | STK38 serine/threonine kinase 38 [ Homo sapiens (human) ] and MOB1B MOB kinase activator 1B [ Homo sapiens (human) ] |
| Official Symbol : | STK38 and MOB1B |
| Synonyms : | STK38 and MOB1B; serine/threonine kinase 38 and MOB kinase activator 1B; NDR; NDR1 and MATS2; MOB4A; MOBKL1A |
| Gene ID : | 11329 and 92597 |
| mRNA Refseq : | NM_007271.4 and NM_173468.4 |
| Protein Refseq : | NP_009202.1 and NP_775739.1 |
| MIM : | 606964 and 609282 |
| UniProt ID : | Q15208 and Q7L9L4 |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket
