Active Recombinant Full Length Human TSSK1B Protein, N-His-tagged

• Cat.No.: TSSK1B-179HFL
• Product Overview: N-terminal 6Histagged, recombinant, human, full length TSSK1, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Testis-specific serine kinases (TSSK1 and TSSK2) are expressed exclusively in testis of sexually mature males. TSSK1 and TSSK2 are absent in released sperms in the lumen of the seminiferous tubules and the epididymis, suggesting they play a role in motility and morphology of spermatozoa. Inhibition of TSSK1 may cause male sterility.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.98mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 526/mg, where one unit of TSSK1, active activity is defined as 1nmol phosphate incorporated into 300μM (KKKVSRSGLYRSPSMPENLNRPR) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 46 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MDDAAVLKRR GYLLGINLGE GSYAKVKSAY 61 SERLKFNVAI KIIDRKKAPA DFLEKFLPRE IEILAMLNHC SIIKTYEIFE TSHGKVYIVM 121 ELAVQGDLLE LIKTRGALHE DEARKKFHQL SLAIKYCHDL DVVHRDLKCD NLLLDKDFNI 181 KLSDFSFSKR CLRDDSGRMA LSKTFCGSPA YAAPEVLQGI PYQPKVYDIW SLGVILYIMV 241 CGSMPYDDSN IKKMLRIQKE HRVNFPRSKH LTGECKDLIY HMLQPDVNRR LHIDEILSHC 301 WMQPKARGSP SVAINKEGES SRGTEPLWTP EPGSDKKSAT KLEPEGEAQP QAQPETKPEG 361 TAMQMSRQSE ILGFPSKPST METEEGPPQQ PPETRAQ
• Purity: Purity 69% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: TSSK1B testis specific serine kinase 1B [ Homo sapiens (human) ]
• Official Symbol: TSSK1B
• Synonyms: TSSK1B; testis specific serine kinase 1B; TSK1; TSSK1; FKSG81; SPOGA4; STK22D
• Gene ID: 83942
• mRNA Refseq: NM_032028.4
• Protein Refseq: NP_114417.1
• MIM: 610709
• UniProt ID: Q9BXA7