Active Recombinant Full Length Human TSSK2 Protein, N-His-tagged

• Cat.No.: TSSK2-180HFL
• Product Overview: N-terminal 6Histagged, recombinant, full length, human TSSK2, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Testis-specific serine kinases (TSSK1 and TSSK2) are expressed exclusively in testis of sexually mature males. TSSK1 and TSSK2 are absent in released sperms in the lumen of the seminiferous tubules and the epididymis, suggesting they play a role in motility and morphology of spermatozoa.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 2.718mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1552U/mg, where one unit of TSSk2 activity is defined as 1nmol phosphate incorporated into 100μM CHKtide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 44.8 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MDDATVLRKK GYIVGINLGK GSYAKVKSAY 61 SERLKFNVAV KIIDRKKTPT DFVERFLPRE MDILATVNHG SIIKTYEIFE TSDGRIYIIM 121 ELGVQGDLLE FIKCQGALHE DVARKMFRQL SSAVKYCHDL DIVHRDLKCE NLLLDKDFNI 181 KLSDFGFSKR CLRDSNGRII LSKTFCGSAA YAAPEVLQSI PYQPKVYDIW SLGVILYIMV 241 CGSMPYDDSD IRKMLRIQKE HRVDFPRSKN LTCECKDLIY RMLQPDVSQR LHIDEILSHS 301 WLQPPKPKAT SSASFKREGE GKYRAECKLD TKTDLRPDHR PDHKLGAKTQ HRLLVVPENE 361 NRMEDRLAET SRAKDHHISG AEVGKAST
• Purity: Purity 74% by SDS-PAGE and Coomassie blue staining. M
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: TSSK2 testis specific serine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: TSSK2
• Synonyms: TSSK2; testis specific serine kinase 2; TSK2; DGS-G; SPOGA2; STK22B
• Gene ID: 23617
• mRNA Refseq: NM_053006.5
• Protein Refseq: NP_443732.3
• MIM: 610710
• UniProt ID: Q96PF2