Active Recombinant Full Length Human TSSK3 Protein, C-His-tagged

• Cat.No.: TSSK3-181HFL
• Product Overview: C-terminal 6His-tagged, recombinant, human TSSK3 full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography followed by size exclusion chromatography.

Specification

• Description: TSSK3 (Testis-specific serine/threonine-protein kinase 3) is a serine/threonine protein kinase that is primarily expressed in the testis, where it plays a role in the regulation of spermatogenesis. TSSK3 has been shown to be involved in the regulation of sperm motility and morphology, and alterations in TSSK3 expression and activity have been linked to male infertility. Research has suggested that TSSK3 may also play a role in cancer. TSSK3 has been shown to be overexpressed in several types of cancer, including breast cancer and prostate cancer, and high levels of TSSK3 expression have been associated with poor prognosis. Additionally, TSSK3 has been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.42mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 729U/mg, where one unit of TSSK3 activity is defined as 1nmol phosphate incorporated into 100μM AMARAASAAALARRR per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 31 kDa
• AA Sequence: 1 MEDFLLSNGY QLGKTIGEGT YSKVKEAFSK KHQRKVAIKV IDKMGGPEEF IQRFLPRELQ 61 IVRTLDHKNI IQVYEMLESA DGKICLVMEL AEGGDVFDCV LNGGPLPESR AKALFRQMVE 121 AIRYCHGCGV AHRDLKCENA LLQGFNLKLT DFGFAKVLPK SHRELSQTFC GSTAYAAPEV 181 LQGIPHDSKK GDVWSMGVVL YVMLCASLPF DDTDIPKMLW QQQKGVSFPT HLSISADCQD 241 LLKRLLEPDM ILRPSIEEVS WHPWLASTHH HHHH
• Purity: Purity 80% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: TSSK3 testis specific serine kinase 3 [ Homo sapiens (human) ]
• Official Symbol: TSSK3
• Synonyms: TSSK3; testis specific serine kinase 3; TSK3; SPOGA3; STK22C; STK22D
• Gene ID: 81629
• mRNA Refseq: NM_052841.4
• Protein Refseq: NP_443073.1
• MIM: 607660
• UniProt ID: Q96PN8