Active Recombinant Full Length Mouse PIK3CB and PIK3R2 Co-expressed Protein, N-His-tagged

• Cat.No.: PIK3CA-PIK3R2-136MFL
• Product Overview: Complex of N-terminal 6His-tagged recombinant mouse p110β full length and untagged, recombinant, mouse p85β full length. Co-expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose.

Specification

• Description: PI3 Kinase plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. It also participates in cellular signaling in response to various growth factors and involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. PI3K regulates invadopodia formation through the PDPK1-AKT1 pathway, and participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others.
• Source: Sf21
• Species: Mouse
• Tag: His
• Protein length: Full Length
• Form: 2.22mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 535U/mg, where one unit of PI 3-Kinase (p110β/p85β) activity is defined as 1nmol phosphatidylinositol 3,4,5-trisphosphate (PIP3) formed per minute at room temperature with a final ATP concentration of 100μM.
• Molecular Mass: p110β MW = 125.5kDa, p85β MW = 81.4kDa
• AA Sequence: p110β amino acid sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MPPAMADNLD IWAVDSQIAS DGAISVDFLL 61 PTGIYIQLEV PREATISYIK QMLWKQVHNY PMFNLLMDID SYMFACVNQT AVYEELEDET 121 RRLCDVRPFL PVLKLVTRSC DPAEKLDSKI GVLIGKGLHE FDALKDPEVN EFRRKMRKFS 181 EAKIQSLVGL SWIDWLKHTY PPEHEPSVLE NLEDKLYGGK LVVAVHFENS QDVFSFQVSP 241 NLNPIKINEL AIQKRLTIRG KEDEASPCDY VLQVSGRVEY VFGDHPLIQF QYIRNCVMNR 301 TLPHFILVEC CKIKKMYEQE MIAIEAAINR NSSNLPLPLP PKKTRVISHI WDNNNPFQIT 361 LVKGNKLNTE ETVKVHVRAG LFHGTELLCK TVVSSEISGK NDHIWNEQLE FDINICDLPR 421 MARLCFAVYA VLDKVKTKKS TKTINPSKYQ TIRKAGKVHY PVAWVNTMVF DFKGQLRSGD 481 VILHSWSSFP DELEEMLNPM GTVQTNPYAE NATALHITFP ENKKQPCYYP PFDKIIEKAA 541 ELASGDSANV SSRGGKKFLA VLKEILDRDP LSQLCENEMD LIWTLRQDCR ENFPQSLPKL 601 LLSIKWNKLE DVAQLQALLQ IWPKLPPREA LELLDFNYPD QYVREYAVGC LRQMSDEELS 661 QYLLQLVQVL KYEPFLDCAL SRFLLERALD NRRIGQFLFW HLRSEVHTPA VSVQFGVILE 721 AYCRGSVGHM KVLSKQVEAL NKLKTLNSLI KLNAVKLSRA KGKEAMHTCL KQSAYREALS 781 DLQSPLNPCV ILSELYVEKC KYMDSKMKPL WLVYSSRAFG EDSVGVIFKN GDDLRQDMLT 841 LQMLRLMDLL WKEAGLDLRM LPYGCLATGD RSGLIEVVST SETIADIQLN SSNVAATAAF 901 NKDALLNWLK EYNSGDDLDR AIEEFTLSCA GYCVASYVLG IGDRHSDNIM VKKTGQLFHI 961 DFGHILGNFK SKFGIKRERV PFILTYDFIH VIQQGKTGNT EKFGRFRQCC EDAYLILRRH 1021 GNLFITLFAL MLTAGLPELT SVKDIQYLKD SLALGKSEEE ALKQFKQKFD EALRESWTTK 1081 VNWMAHTVRK DYRS
  p85β amino acid sequence:
  1 MAGAEGFQYR AVYPFRRERP EDLELLPGDL LVVSRVALQA LGVADGGERC PHNVGWMPGF
  61 NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP LDGSSESGHI LPDLAEQFSP
  121 PDPAPPILVK LVEAIEQAEL DSECYSKPEL PATRTDWSLS DLEQWDRTAL YDAVKGFLLA
  181 LPAAVVTPEA AAEAYRALRE VAGPVGLVLE PPTLPLHQAL TLRFLLQHLG RVARRAPSPD
  241 TAVHALASAF GPLLLRIPPS GGEGDGSEPV PDFPVLLLER LVQEHVEEQD AAPPALPPKP
  301 SKAKPAPTAL ANGGSPPSLQ DAEWYWGDIS REEVNERLRD TPDGTFLVRD ASSKIQGEYT
  361 LTLRKGGNNK LIKVFHRDGH YGFSEPLTFC SVVELISHYR HESLAQYNAK LDTRLLYPVS
  421 KYQQDQVVKE DSVEAVGAQL KVYHQQYQDK SREYDQLYEE YTRTSQELQM KRTAIEAFNE
  481 TIKIFEEQGQ TQEKCSKEYL ERFRREGNEK EMQRILLNSE RLKSRIAEIH ESRTKLEQDL
  541 RAQASDNREI DKRMNSLKPD LMQLRKIRDQ YLVWLTQKGA RQRKINEWLG IKNETEDQYS
  601 LMEDEDALPH HEERTWYVGK INRTQAEEML SGKRDGTFLI RESSQRGCYA CSVVVDGDTK
  661 HCVIYRTATG FGFAEPYNLY GSLKELVLHY QHASLVQHND ALTVTLAHPV RAPGPGPPSA
  721 AR
• Purity: Purity (p110β and p85β combined) 95% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: Pik3cb phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta [ Mus musculus (house mouse) ] and Pik3r2 phosphoinositide-3-kinase regulatory subunit 2 [ Mus musculus (house mouse) ]
• Official Symbol: PIK3CB and PIK3R2
• Synonyms: PIK3CB and PIK3R2; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta and phosphoinositide-3-kinase regulatory subunit 2; p110beta; 1110001J02Rik and p85beta
• Gene ID: 74769 and 18709
• mRNA Refseq: NM_029094.3 and NM_008841.3
• Protein Refseq: NP_083370.2 and NP_032867.2
• UniProt ID: Q8BTI9 and O08908