Active Recombinant Full Length Mouse PIK3CD and PIK3R1 Co-expressed Protein, N-His-tagged

• Cat.No.: PIK3CD-PIK3R1-142MFL
• Product Overview: Complex of N-terminal 6His-tagged recombinant mouse p110delta full length and untagged, recombinant, mouse p85alpha full length. Co-expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose.

Specification

• Description: PI3 Kinase plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. It also participates in cellular signaling in response to various growth factors and involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. PI3K regulates invadopodia formation through the PDPK1-AKT1 pathway, and participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others.
• Source: Sf21
• Species: Mouse
• Tag: His
• Protein length: Full Length
• Form: 0.774mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 343U/mg, where one unit of PI 3-Kinase (p110δ/p85α) activity is defined as 1nmol phosphatidylinositol 3,4,5-trisphosphate (PIP3) formed per minute at room temperature with a final ATP concentration of 100μM.
• Molecular Mass: p110δ MW = 124kDa, p85α MW = 84kDa
• AA Sequence: p110δ amino acid sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRQMPPG VDCPMEFWTK EESQSVVVDF 61 LLPTGVYLNF PVSRNANLST IKQVLWHRAQ YEPLFHMLSD PEAYVFTCVN QTAEQQELED 121 EQRRLCDIQP FLPVLRLVAR EGDRVKKLIN SQISLLIGKG LHEFDSLRDP EVNDFRTKMR 181 QFCEEAAAHR QQLGWVEWLQ YSFPLQLEPS ARGWRAGLLR VSNRALLVNV KFEGSEESFT 241 FQVSTKDMPL ALMACALRKK ATVFRQPLVE QPEEYALQVN GRHEYLYGNY PLCHFQYICS 301 CLHSGLTPHL TMVHSSSILA MRDEQSNPAP QVQKPRAKPP PIPAKKPSSV SLWSLEQPFS 361 IELIEGRKVN ADERMKLVVQ AGLFHGNEML CKTVSSSEVN VCSEPVWKQR LEFDISVCDL 421 PRMARLCFAL YAVVEKAKKA RSTKKKSKKA DCPIAWANLM LFDYKDQLKT GERCLYMWPS 481 VPDEKGELLN PAGTVRGNPN TESAAALVIY LPEVAPHPVY FPALEKILEL GRHGERGRIT 541 EEEQLQLREI LERRGSGELY EHEKDLVWKM RHEVQEHFPE ALARLLLVTK WNKHEDVAQM 601 LYLLCSWPEL PVLSALELLD FSFPDCYVGS FAIKSLRKLT DDELFQYLLQ LVQVLKYESY 661 LDCELTKFLL GRALANRKIG HFLFWHLRSE MHVPSVALRF GLIMEAYCRG STHHMKVLMK 721 QGEALSKLKA LNDFVKVSSQ KTTKPQTKEM MHMCMRQETY MEALSHLQSP LDPSTLLEEV 781 CVEQCTFMDS KMKPLWIMYS SEEAGSAGNV GIIFKNGDDL RQDMLTLQMI QLMDVLWKQE 841 GLDLRMTPYG CLPTGDRTGL IEVVLHSDTI ANIQLNKSNM AATAAFNKDA LLNWLKSKNP 901 GEALDRAIEE FTLSCAGYCV ATYVLGIGDR HSDNIMIRES GQLFHIDFGH FLGNFKTKFG 961 INRERVPFIL TYDFVHVIQQ GKTNNSEKFE RFRGYCERAY TILRRHGLLF LHLFALMRAA 1021 GLPELSCSKD IQYLKDSLAL GKTEEEALKH FRVKFNEALR ESWKTKVNWL AHNVSKDNRQ
  p85α amino acid sequence:
  1 MSAEGYQYRA LYDYKKEREE DIDLHLGDIL TVNKGSLVAL GFSDGQEARP EDIGWLNGYN
  61 ETTGERGDFP GTYVEYIGRK RISPPTPKPR PPRPLPVAPG SSKTEADTEQ QALPLPDLAE
  121 QFAPPDVAPP LLIKLLEAIE KKGLECSTLY RTQSSSNPAE LRQLLDCDAA SVDLEMIDVH
  181 VLADAFKRYL ADLPNPVIPV AVYNEMMSLA QELQSPEDCI QLLKKLIRLP NIPHQCWLTL
  241 QYLLKHFFKL SQASSKNLLN ARVLSEIFSP VLFRFPAASS DNTEHLIKAI EILISTEWNE
  301 RQPAPALPPK PPKPTTVANN SMNNNMSLQD AEWYWGDISR EEVNEKLRDT ADGTFLVRDA
  361 STKMHGDYTL TLRKGGNNKL IKIFHRDGKY GFSDPLTFNS VVELINHYRN ESLAQYNPKL
  421 DVKLLYPVSK YQQDQVVKED NIEAVGKKLH EYNTQFQEKS REYDRLYEEY TRTSQEIQMK
  481 RTAIEAFNET IKIFEEQCQT QERYSKEYIE KFKREGNEKE IQRIMHNHDK LKSRISEIID
  541 SRRRLEEDLK KQAAEYREID KRMNSIKPDL IQLRKTRDQY LMWLTQKGVR QKKLNEWLGN
  601 ENTEDQYSLV EDDEDLPHHD EKTWNVGSSN RNKAENLLRG KRDGTFLVRE SSKQGCYACS
  661 VVVDGEVKHC VINKTATGYG FAEPYNLYSS LKELVLHYQH TSLVQHNDSL NVTLAYPVYA
  721 QQRR
• Purity: Purity (p110δ and p85α combined) 76% by SDSPAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: Pik3cd phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta [ Mus musculus (house mouse) ] and Pik3r1 phosphoinositide-3-kinase regulatory subunit 1 [ Mus musculus (house mouse) ]
• Official Symbol: PIK3CD and PIK3R1
• Synonyms: PIK3CD and PIK3R1; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta and phosphoinositide-3-kinase regulatory subunit 1; p110delta; 2410099E07Rik; 2610208K16Rik and PI3K; p50alpha; p55alpha; p85alpha
• Gene ID: 18707 and 18708
• mRNA Refseq: NM_008840.3 and NM_001024955.2
• Protein Refseq: NP_032866.2 and NP_001020126.1
• UniProt ID: O35904 and P26450