Active Recombinant Human ENPP2 protein, His-tagged

• Cat.No.: ENPP2-03H
• Product Overview: Recombinant Human ENPP2 protein(Asp 49 - Ile 863), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is >8,000 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus.The protein has a calculated MW of 94.5 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE).
• Protein Length: Asp49-Ile863
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ENPP2 ectonucleotide pyrophosphatase/phosphodiesterase 2 [ Homo sapiens ]
• Official Symbol: ENPP2
• Synonyms: ENPP2; ectonucleotide pyrophosphatase/phosphodiesterase 2; PDNP2; ectonucleotide pyrophosphatase/phosphodiesterase family member 2; ATX; autotaxin; PD IALPHA; E-NPP 2; autotaxin-t; plasma lysophospholipase D; extracellular lysophospholipase D; phosphodiesterase I/nucleotide pyrophosphatase 2; NPP2; ATX-X; LysoPLD; AUTOTAXIN; PD-IALPHA; FLJ26803
• Gene ID: 5168
• mRNA Refseq: NM_001040092
• Protein Refseq: NP_001035181
• MIM: 601060
• UniProt ID: Q13822