Active Recombinant Human HASPIN(471-end) Protein, N-His-tagged

• Cat.No.: HASPIN-96H
• Product Overview: N-terminal 6Histagged, recombinant, human Haspin, amino acids 471–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: HASPIN (Histone H3 Associated Protein Kinase) is a Protein Coding gene. An important paralog of this gene is IRAK1. This protein is a Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph) during mitosis. May act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 471-end
• Form: 2.033mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1340U/mg, where one unit of Haspin activity is defined as 1nmol phosphate incorporated into 500μM (RARTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 41.1 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF GPVPFSHCLP TEKLQRCEKI GEGVFGEVFQ 61 TIADHTPVAI KIIAIEGPDL VNGSHQKTFE EILPEIIISK ELSLLSGEVC NRTEGFIGLN 121 SVHCVQGSYP PLLLKAWDHY NSTKGSANDR PDFFKDDQLF IVLEFEFGGI DLEQMRTKLS 181 SLATAKSILH QLTASLAVAE ASLRFEHRDL HWGNVLLKKT SLKKLHYTLN GKSSTIPSCG 241 LQVSIIDYTL SRLERDGIVV FCDVSMDEDL FTGDGDYQFD IYRLMKKENN NRWGEYHPYS 301 NVLWLHYLTD KMLKQMTFKT KCNTPAMKQI KRKIQEFHRT MLNFSSATDL LCQHSLFK
• Purity: Purity 62.8% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: HASPIN histone H3 associated protein kinase [ Homo sapiens (human) ]
• Official Symbol: HASPIN
• Synonyms: HASPIN; histone H3 associated protein kinase; GSG2
• Gene ID: 83903
• mRNA Refseq: NM_031965.2
• Protein Refseq: NP_114171.2
• MIM: 609240
• UniProt ID: Q8TF76