Active Recombinant Human HIPK2(165-564) Protein, N-His-tagged

• Cat.No.: HIPK2-101H
• Product Overview: N-terminal 6Histagged, recombinant, human HIPK2, amino acids 165–564, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose.

Specification

• Description: Homeodomain-interacting protein kinase 2 (HIPK2), a serine/threonine kinase, has been described as a member of a family of nuclear kinases that act as co-repressors for homeodomain transcription factors. HIPK2 can activate p53-mediated transcriptional regulation of MDM2 and antagonize Myc/Ras-induced transformation. HIPK2 may play an important role in the regulation of p53 apoptotic function. Inhibition of HIPK2 may inhibit apoptosis and facilitate tumorigenesis. GenBank AF326592. The recombinant protein contains the amino acid substitution I471N (native coordinates) with respect to GenBank AF326592. This conflict is reported in GenBank accession nos. AF207702, AF208291, NM022740.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 165-564 aa
• Form: 2.11mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 2066U/mg, where one unit of HIPK2, active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 49.6kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF TATTSTATSK NSGSNSEGDY QLVQHEVLCS 61 MTNTYEVLEF LGRGTFGQVV KCWKRGTNEI VAIKILKNHP SYARQGQIEV SILARLSTES 121 ADDYNFVRAY ECFQHKNHTC LVFEMLEQNL YDFLKQNKFS PLPLKYIRPV LQQVATALMK 181 LKSLGLIHAD LKPENIMLVD PSRQPYRVKV IDFGSASHVS KAVCSTYLQS RYYRAPEIIL 241 GLPFCEAIDM WSLGCVIAEL FLGWPLYPGA SEYDQIRYIS QTQGLPAEYL LSAGTKTTRF 301 FNRDTDSPYP LWRLKTPDDH EAETGIKSKE ARKYIFNCLD DMAQVNMTTD LEGSDMLVEK 361 ADRREFIDLL KKMLTIDADK RITPIETLNH PFVTMTHLLD FPHSTHVKSC FQNMEICKRR 421 VNMYDTVNQS
• Purity: Purity 94% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: HIPK2 homeodomain interacting protein kinase 2 [ Homo sapiens (human) ]
• Official Symbol: HIPK2
• Synonyms: HIPK2; homeodomain interacting protein kinase 2; PRO0593
• Gene ID: 28996
• mRNA Refseq: NM_022740.5
• Protein Refseq: NP_073577.3
• MIM: 606868
• UniProt ID: Q9H2X6