Active Recombinant Human PRKCE(2-end) Protein, N-His-tagged

• Cat.No.: PRKCE-199H
• Product Overview: N-terminal 6Histagged, recombinant PKC epsilon, amino acids 2–end, expressed in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Protein kinase Ce (PKCe) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphosphate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 2-end
• Form: 0.126mg/ml of enzyme in 20mM Tris/HCl pH 7.5, 1mM EGTA, 1mM EDTA, 0.02% Triton X-100, 5% glycerol, 1mM PMSF, 10mM benzamidine, 1mM DTT. Frozen solution.
• Bio-activity: 2420U/mg, where one unit of PKC epsilon, active activity is defined as 1nmol phosphate incorporated into 50μM PKCtide (ERMRPRKRQGSVRRRV) in the presence of lipid activator per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 84.6 kDa
• AA Sequence: 1 MHHHHHHVVF NGLLKIKICE AVSLKPTAWS LRHAVGPRPQ TFLLDPYIAL NVDDSRIGQT 61 ATKQKTNSPA WHDEFVTDVC NGRKIELAVF HDAPIGYDDF VANCTIQFEE LLQNGSRHFE 121 DWIDLEPEGR VYVIIDLSGS SGEAPKDNEE RVFRERMRPR KRQGAVRRRV HQVNGHKFMA 181 TYLRQPTYCS HCRDFIWGVI GKQGYQCQVC TCVVHKRCHE LIITKCAGLK KQETPDQVGS 241 QRFSVNMPHK FGIHNYKVPT FCDHCGSLLW GLLRQGLQCK VCKMNVHRRC ETNVAPNCGV 301 DARGIAKVLA DLGVTPDKIT NSGQRRKKLI AGAESPQPAS GSSPSEEDRS KSAPTSPCDQ 361 EIKELENNIR KALSFDNRGE EHRAASSPDG QLMSPGENGE VRQGQAKRLG LDEFNFIKVL 421 GKGSFGKVML AELKGKDEVY AVKVLKKDVI LQDDDVDCTM TEKRILALAR KHPYLTQLYC 481 CFQTKDRLFF VMEYVNGGDL MFQIQRSRKF DEPRSRFYAA EVTSALMFLH QHGVIYRDLK 541 LDNILLDAEG HCKLADFGMC KEGILNGVTT TTFCGTPDYI APEILQELEY GPSVDWWALG 601 VLMYEMMAGQ PPFEADNEDD LFESILHDDV LYPVWLSKEA VSILKAFMTK NPHKRLGCVA 661 SQNGEDAIKQ HPFFKEIDWV LLEQKKIKPP FKPRIKTKRD VNNFDQDFTR EEPVLTLVDE 721 AIVKQINQEE FKGFSYFGED LMP
• Purity: Purity 96.3% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PRKCE protein kinase C epsilon [ Homo sapiens (human) ]
• Official Symbol: PRKCE
• Synonyms: PRKCE; protein kinase C epsilon; PKCE; nPKC-epsilon
• Gene ID: 5581
• mRNA Refseq: NM_005400.3
• Protein Refseq: NP_005391.1
• MIM: 176975
• UniProt ID: Q02156