Active Recombinant Human SIK2(1-276) Protein, N-GST-tagged

• Cat.No.: SIK2-228H
• Product Overview: N-terminal GST-tagged, recombinant, human SIK2 amino acids 1-276 expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: SIK2 phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. It inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 1-276 aa
• Form: 1.98 mg/ml of enzyme in 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 370 U/mg, where one unit of SIK2 activity is defined as 1 nmol phosphate incorporated into 100 μM KKKVSRSGLYRSPSMPENLNRPR per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 59 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFM VMADGPRHLQ 241 RGPVRVGFYD IEGTLGKGNF AVVKLGRHRI TKTEVAIKII DKSQLDAVNL EKIYREVQIM 301 KMLDHPHIIK LYQVMETKSM LYLVTEYAKN GEIFDYLANH GRLNESEARR KFWQILSAVD 361 YCHGRKIVHR DLKAENLLLD NNMNIKIADF GFGNFFKSGE LLATWCGSPP YAAPEVFEGQ 421 QYEGPQLDIW SMGVVLYVLV CGALPFDGPT LPILRQRVLE GRFRIPYFMS EDCEHLIRRM 481 LVLDPSKRLT IAQIKEHKWM LIEVP
• Purity: Purity 76% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: SIK2 salt inducible kinase 2 [ Homo sapiens (human) ]
• Official Symbol: SIK2
• Synonyms: SIK2; salt inducible kinase 2; QIK; SIK-2; SNF1LK2; LOH11CR1I
• Gene ID: 23235
• mRNA Refseq: NM_015191.3
• Protein Refseq: NP_056006.1
• MIM: 608973
• UniProt ID: Q9H0K1