Active Recombinant Human TAOK2(1-320) Protein, N-His-tagged

• Cat.No.: TAOK2-238H
• Product Overview: N-terminal 6Histagged, recombinant, TAO2, amino acids 1–320, expressed in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: TAOK2 (TAO Kinase 2) is a serine/threonine protein kinase that is involved in many different processes, including, cell signaling, microtubule organization and stability, and apoptosis. TAO2 is involved in different processes such as membrane blebbing and apoptotic bodies formation DNA damage response and MAPK14/p38 MAPK stress-activated MAPK cascade. In response to DNA damage, TAO2 is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-320 aa
• Form: 0.751mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 641U/mg, where one unit of TOA2, active activity is defined as 1nmol phosphate incorporated into 0.8mg/ml myelin basic per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 40.1 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MPAGGRAGSL KDPDVAELFF KDDPEKLFSD 61 LREIGHGSFG AVYFARDVRN SEVVAIKKMS YSGKQSNEKW QDIIKEVRFL QKLRHPNTIQ 121 YRGCYLREHT AWLVMEYCLG SASDLLEVHK KPLQEVEIAA VTHGALQGLA YLHSHNMIHR 181 DVKAGNILLS EPGLVKLGDF GSASIMAPAN SFVGTPYWMA PEVILAMDEG QYDGKVDVWS 241 LGITCIELAE RKPPLFNMNA MSALYHIAQN ESPVLQSGHW SEYFRNFVDS CLQKIPQDRP 301 TSEVLLKHRF VLRERPPTVI MDLIQRTKDA VRELDNLQYR KMKKILFQEA
• Purity: Purity 66.5% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: TAOK2 TAO kinase 2 [ Homo sapiens (human) ]
• Official Symbol: TAOK2
• Synonyms: TAOK2; TAO kinase 2; PSK; PSK1; TAO1; TAO2; MAP3K17; Tao2beta; PSK1-BETA
• Gene ID: 9344
• mRNA Refseq: NM_004783.4
• Protein Refseq: NP_004774.1
• MIM: 613199
• UniProt ID: Q9UL54