Recombinant Human Tubulin Folding Cofactor A

• Cat.No.: TBCA-6925H
• Product Overview: Recombinant human TBCA protein was expressed in E. coli and purified by using conventional chromatography techniques. MW: 12.8 kDa.

Specification

• Description: TBCA is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest.
• Source: E. coli
• Species: Human
• Tag: N/A
• Form: Liquid. In 20 mM Tris-HCl buffer pH 7.5 containing 1 mM DTT, 10% glycerol.
• Molecular Mass: 12.8 kDa (108aa), confirmed by MALDI-TOF.
• Protein length: 1-108aa
• AA Sequence: MADPRVRQIK IKTGVVKRLV KEKVMYEKEA KQQEEKIEKM RAEDGENYDI KKQAEILQES RMMIPDCQRR LEAAYLDLQR ILENEKDLEE AEEYKEARLV LDSVKLEA
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TBCA tubulin folding cofactor A [ Homo sapiens ]
• Official Symbol: TBCA
• Synonyms: TBCA; tubulin folding cofactor A; tubulin specific chaperone a; tubulin-specific chaperone A; CFA; chaperonin cofactor A; TCP1-chaperonin cofactor A; tubulin-folding cofactor A
• Gene ID: 6902
• mRNA Refseq: NM_004607
• Protein Refseq: NP_004598
• MIM: 610058
• UniProt ID: O75347
• Chromosome Location: 5q14.1
• Pathway: Metabolism of proteins, organism-specific biosystem; Post-chaperonin tubulin folding pathway, organism-specific biosystem; Protein folding, organism-specific biosystem
• Function: chaperone binding; unfolded protein binding