||Recombinant human STAR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||STAR, also known as steroidogenic acute regulatory protein, appears to mediate the rapid increase in pregnenolone synthesis stimulated by tropic hormones. It increases pregnenolone synthesis more than four-fold and a major STAR transcript of 1.6 kb is found in ovary and testis. During ongoing growth and differentiation of the follicle of the ovary, the immunoreactivity of it tends to shift from the granulosa cells of early antral follicles to the theca cell layers in the adult.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT
||27.1 kDa (243aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||1.0 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MEETLYSDQE LAYLQQGEEA MQKALGILSN QEGWKKESQQ DNGDKVMSKV VPDVGKVFRL EVVVDQPMER LYEELVERME AMGEWNPNVK EIKVLQKIGK DTFITHELAA EAAGNLVGPR DFVSVRCAKR RGSTCVLAGM ATDFGNMPEQ KGVIRAEHGP TCMVLHPLAG SPSKTKLTWL LSIDLKGWLP KSIINQVLSQ TQVDFANHLR KRLESHPASE ARC
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Metabolism of lipids and lipoproteins; Metabolism of steroid hormones and vitamins A and D; Pregnenolone biosynthesis