"PHOSPHO1" Related Products

Recombinant Human PHOSPHO1, His-tagged

Cat. No.: PHOSPHO1-1028H
Product Overview: Total 295 AA. MW: 31.3 kDa (calculated). N-Terminal His-tag 14 AA (highlighted).
Description: PHOSPHO1 is an ethanolamine derivative that is used to construct sphingomyelins.
Source: E. coli
Species: Human
Form: Filtered (0, 4 μm) and lyophilized in 0.5 mg/mL in 0.03 M Acetate buffer, pH 4
Research topic: Energy metabolism and body weight regulation
Purity: >95%
Reconstitution: Add 0.1 M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 μg/mL. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Applications: Western blotting
Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
Sequences of amino acids: MRGSHHHHHH GMASMSGCFP VSGLRCLSRD GRMAAQGAPR FLLTFDFDET IVDENSDDSI VRAAPGQRLP ESLRATYREG FYNEYMQRVF KYLGEQGVRP RDLSAIYEAI PLSPGMSDLL QFVAKQGACF EVILISDANT FGVESSLRAA GHHSLFRRIL SNPSGPDARG LLALRPFHTH SCARCPANMC KHKVLSDYLR ERAHDGVHFE RLFYVGDGAN DFCPMGLLAG GDVAFPRRGY PMHRLIQEAQ KAEPSSFRAS VVPWETAADV RLHLQQVLKS C
Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
Gene Name: PHOSPHO1 phosphatase, orphan 1 [ Homo sapiens ]
Official Symbol: PHOSPHO1
Synonyms: PHOSPHO1; phosphatase, orphan 1; phosphoethanolamine/phosphocholine phosphatase; OTTHUMP00000220006; OTTHUMP00000220008; OTTHUMP00000220009
Gene ID: 162466
mRNA Refseq: NM_001143804
Protein Refseq: NP_001137276
UniProt ID: Q8TCT1
Chromosome Location: 17q21
Pathway: Glycerophospholipid metabolism; Metabolic pathways
Function: hydrolase activity; metal ion binding; phosphoethanolamine/phosphocholine phosphatase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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