Recombinant Human MLEC, His-tagged

• Cat.No.: MLEC-15872H
• Product Overview: Recombinant human MLEC protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: MLEC belongs to the malectin family and is localized on endoplasmic reticulum membrane. This protein is a carbohydrate-binding protein with a strong ligand preference for Glc2-N-glycan and may play a role in the early steps of protein N-glycosylation.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 29.1kDa (264aa), confirmed by MALDI-TOF
• Protein length: 29-269aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSPGLGVAG VAGAAGAGLP ESVIWAVNAG GEAHVDVHGI HFRKDPLEGR VGRASDYGMK LPILRSNPED QILYQTERYN EETFGYEVPI KEEGDYVLVL KFAEVYFAQS QQKVFDVRLN GHVVVKDLDI FDRVGHSTAH DEIIPMSIRK GKLSVQGEVS TFTGKLYIEF VKGYYDNPKV CALYIMAGTV DDVPKLQPHP GLEKKEEEEE EEEYDEGSNL KKQTNKNRVQ SGPRTPNPYA SDNS
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MLEC malectin [ Homo sapiens ]
• Official Symbol: MLEC
• Synonyms: MLEC; malectin; KIAA0152
• Gene ID: 9761
• mRNA Refseq: NM_014730
• Protein Refseq: NP_055545
• MIM: 613802
• UniProt ID: Q14165
• Chromosome Location: 12q24.31
• Pathway: Asparagine N-linked glycosylation, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; N-glycan trimming in the ER and Calnexin/Calreticulin cycle, organism-specific biosystem; Post-translational protein modification, organism-specific biosystem
• Function: carbohydrate binding