Recombinant Human EXOSC8, His-tagged

• Cat.No.: EXOSC8-136H
• Product Overview: EXOSC8, 1-276aa, Human, His tag, E.coli

Specification

• Description: EXOSC8, also known as exosome component 8 and transcription factor 4, is a part of the exosome complex. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3" untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC8 binds to ARE-containing RNAs. Recombinant human EXOSC8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 50% glycerol 0.2M NaCl,1mM DTT
• Molecular Mass: 32.4 kDa (299aa) confirmed by MALDI-TOF
• Protein length: 1-276aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAGFKT VEPLEYYRRF LKENCRPDGR ELGEFRTTTV NIGSISTADG SALVKLGNTT VICGVKAEFA APSTDAPDKG YVVPNVDLPP LCSSRFRSGP PGEEAQVASQ FIADVIENSQ IIQKEDLCIS PGKLVWVLYC DLICLDYDGN ILDACTFALL AALKNVQLPE VTINEETALA EVNLKKKSYL NIRTHPVATS FAVFDDTLLI VDPTGEEEHL ATGTLTIVMD EEGKLCCLHK PGGSGLTGAK LQDCMSRAVT RHKEVKKLMD EVIKSMKPK
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: EXOSC8 exosome component 8 [ Homo sapiens ]
• Official Symbol: EXOSC8
• Synonyms: EXOSC8; exosome component 8; exosome complex component RRP43; bA421P11.3; CBP interacting protein 3; CIP3; EAP2; OIP2; Opa interacting protein 2; p9; RRP43; Rrp43p; OIP-2; CBP-interacting protein 3; opa-interacting protein 2; exosome complex exonuclease RRP43; ribosomal RNA-processing protein 43; RP11-421P11.3
• Gene ID: 11340
• mRNA Refseq: NM_181503
• Protein Refseq: NP_852480
• MIM: 606019
• UniProt ID: Q96B26
• Chromosome Location: 13q13.1
• Pathway: Activation of Genes by ATF4, organism-specific biosystem; Deadenylation-dependent mRNA decay, organism-specific biosystem; Destabilization of mRNA by Butyrate Response Factor 1 (BRF1), organism-specific biosystem; Destabilization of mRNA by KSRP, organism-specific biosystem; Destabilization of mRNA by Tristetraprolin (TTP), organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem
• Function: AU-rich element binding; RNA binding; NOT exoribonuclease activity; protein binding