"PPIL3" Related Products

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Recombinant Human PPIL3 293 Cell Lysate

Cat.No.: PPIL3-2966HCL
Description: Antigen standard for peptidylprolyl isomerase (cyclophilin)-like 3 (PPIL3), transcript variant PPIL3c is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PPIL3 peptidylprolyl isomerase (cyclophilin)-like 3 [ Homo sapiens ]
Official Symbol: PPIL3
Synonyms: PPIL3; peptidylprolyl isomerase (cyclophilin)-like 3; peptidyl-prolyl cis-trans isomerase-like 3; Cyclophilin J; CyPJ; PPIase; cyclophilin J; rotamase PPIL3; PPIase-like protein 3; cyclophilin-like protein 3; cyclophilin-like protein PPIL3; peptidylprolyl cis-trans isomerase-like protein 3; CYPJ;
Gene ID: 53938
mRNA Refseq: NM_032472
Protein Refseq: NP_115861
UniProt ID: Q9H2H8
Chromosome Location: 2q33.1
Function: isomerase activity; peptidyl-prolyl cis-trans isomerase activity; protein binding;

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