"CYBA" Related Products

Recombinant Human CYBA 293 Cell Lysate

Cat.No.: CYBA-7139HCL
Description: Antigen standard for cytochrome b-245, alpha polypeptide (CYBA) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: CYBA cytochrome b-245, alpha polypeptide [ Homo sapiens ]
Official Symbol: CYBA
Synonyms: CYBA; cytochrome b-245, alpha polypeptide; cytochrome b-245 light chain; flavocytochrome b 558 alpha polypeptide; p22 PHOX; p22phox; cytochrome b light chain; p22 phagocyte B-cytochrome; cytochrome b(558) alpha chain; cytochrome b558 subunit alpha; cytochrome b(558) alpha-subunit; cytochrome b, alpha polypeptide; flavocytochrome b-558 alpha polypeptide; neutrophil cytochrome b 22 kDa polypeptide; superoxide-generating NADPH oxidase light chain subunit; p22-PHOX; FLJ43590; FLJ99071;
Gene ID: 1535
mRNA Refseq: NM_000101
Protein Refseq: NP_000092
UniProt ID: P13498
Chromosome Location: 16q24
Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing-Cross presentation, organism-specific biosystem; Class I MHC mediated antigen processing and presentation, organism-specific biosystem; Cross-presentation of particulate exogenous antigens (phagosomes), organism-specific biosystem; Immune System, organism-specific biosystem; Leishmaniasis, organism-specific biosystem;
Function: SH3 domain binding; electron carrier activity; electron carrier activity; heme binding; metal ion binding; oxidoreductase activity; protein binding; protein heterodimerization activity; contributes_to superoxide-generating NADPH oxidase activity;

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.