Active Recombinant Human JOSD1, His-tagged

• Cat.No.: JOSD1-175H
• Product Overview: Recombinant human JOSD1 (amino acid residues 1-202), fused with N-terminal His, was expressed in E.coli.

Specification

• Description: JOSD1 is a cysteine protease and a member of the Machado-Joseph Domain (MJD) enzyme family. Cloning of the human gene was first described by Nomura et al. (1994). The Josephin domain is a conserved cysteine protease domain found in four human deubiquitylating enzymes: ataxin-3, the ataxin-3-like protein (Ataxin-3L), Josephin-1 (JOSD1), and Josephin-2 (JOSD2). Two of the human Josephin proteins, ataxin-3 and ataxin-3L, each contain a single Josephin domain at their N-terminus plus a flexible C-terminal domain of comparable length. JOSD1 and JOSD2, on the other hand, are each composed solely of a single Josephin domain (Weeks et al., 2011).
• Source: E. coli
• Species: Human
• Tag: His
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: Deubiquitylase Enzyme Assay: The activity of His-JOSD1 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-JOSD1 was compared confirming the deubiquitylating activity of His-JOSD1.
• Molecular Mass: ~26 kDa
• Purity: >98% by SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: JOSD1 Josephin domain containing 1 [ Homo sapiens ]
• Official Symbol: JOSD1
• Synonyms: JOSD1; Josephin domain containing 1; Josephin-1; KIAA0063; josephin domain-containing 1; dJ508I15.2
• Gene ID: 9929
• mRNA Refseq: NM_014876
• Protein Refseq: NP_055691
• UniProt ID: Q15040
• Chromosome Location: 22q13.1
• Function: omega peptidase activity; peptidase activity