Recombinant Human POMC, His-tagged

• Cat.No.: POMC-30690TH
• Product Overview: Recombinant full length Human POMC with an N terminal His tag; 262aa, 28.9kDa inclusive of tag.

Specification

• Description: This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.
• Protein length: 241 amino acids
• Conjugation: HIS
• Molecular Weight: 28.900kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 50% Glycerol, 0.2M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMWCLE SSQCQDLTTESNLLECIRACKPDLS AETPMFPGNGDEQPLTENPRKYVMG HFRWDRFGRRNSSSSGSSGAGQKRE DVSAGEDCGPLPEGGPEPRSDGAKP GPREGKRSYSMEHFRWGKPVGKKRR PVKVYPNGAEDESAEAFPLEFKREL TGQRLREGDGPDGPADDGAGAQADL EHSLLVAAEKKDEGPYRMEHFRWGS PPKDKRYGGFMTSEKSQTPLVTLFK NAIIKNAYKKGE

Gene Information

• Gene Name: POMC proopiomelanocortin [ Homo sapiens ]
• Official Symbol: POMC
• Synonyms: POMC; proopiomelanocortin; pro-opiomelanocortin; ACTH; adrenocorticotropic hormone; adrenocorticotropin; alpha melanocyte stimulating hormone; beta endorphin; beta lipotropin; beta melanocyte stimulating hormone; CLIP; LPH; MSH; NPP; POC
• Gene ID: 5443
• mRNA Refseq: NM_000939
• Protein Refseq: NP_000930
• MIM: 176830
• Uniprot ID: P01189
• Chromosome Location: 2p23
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Androgen biosynthesis, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem
• Function: G-protein coupled receptor binding; hormone activity; hormone activity; receptor binding; type 1 melanocortin receptor binding